PMID: 7547235Oct 1, 1995Paper

MDM2 overexpression is rare in ovarian carcinoma irrespective of TP53 mutation status

British Journal of Cancer
W D FoulkesI G Campbell

Abstract

Somatic mutations in TP53 are seen in many human cancers. In addition, the protein product of the wild-type TP53 can be sequestered by the protein MDM2 (murine double minute 2). This protein is commonly overexpressed in human sarcomas and gliomas, usually as a result of gene amplification. In this study, 43 ovarian carcinomas (OCs) were analysed for aberrations in the TP53 gene by immunohistochemistry (IHC), loss of heterozygosity (LOH) or mutation analysis. The MDM2 gene and its product was studied by Southern blotting and IHC. Over 50% of the OCs studied showed mutations in TP53 by either direct sequencing (19/36, 53%), positive IHC (23,43, 53%) or both, whereas 0/32 had amplification of MDM2 and only 1/37 tumours had positive IHC using the anti-MDM2 antibody IF-2. The solitary example of positive IHC in this series was seen in a mixed müllerian tumour with sarcomatous differentiation and was not accompanied by MDM2 DNA amplification. These results support previous data showing that around 50% of OCs have mutations in TP53 and in addition, suggest that MDM2 is not amplified in OC, but the presence of sarcomatous features in mixed müllerian tumours may result in positive immunohistochemistry with IF-2.

Citations

Feb 27, 2008·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·E J Nam, Y T Kim
Nov 25, 2000·Breast Cancer : the Journal of the Japanese Breast Cancer Society·M ToiT Tominaga
May 27, 2003·Molecular Carcinogenesis·Maria I PatrikisIan G Campbell
Sep 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michael OverholtzerArnold J Levine
May 20, 2016·Cold Spring Harbor Perspectives in Medicine·Jonathan D OlinerSean Caenepeel
Aug 16, 2006·Annals of Human Genetics·A Dansonka-MieszkowskaJ Kupryjańczyk
Aug 22, 1997·International Journal of Cancer. Journal International Du Cancer·B TannerR Becker
Nov 24, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M BaekelandtR Holm
Jun 17, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ian G CampbellDavid Y H Choong
Aug 2, 2003·Gynecologic Oncology·Ruo-Ran Mi, Hong Ni

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