MDM2 Overexpression Modulates the Angiogenesis-Related Gene Expression Profile of Prostate Cancer Cells

Cells
Thiagarajan VenkatesanAppu Rathinavelu

Abstract

The Murine Double Minute 2 (MDM2) amplification or overexpression has been found in many tumors with high metastatic and angiogenic ability. Our experiments were designed to explore the impact of MDM2 overexpression, specifically on the levels of angiogenesis-related genes, which can also play a major role in tumor propagation and increase its metastatic potential. In the present study, we have used the human angiogenesis RT² profiler PCR array to compare the gene expression profile between LNCaP and LNCaP-MST (MDM2 transfected) prostate cancer cells, along with LNCaP-MST cells treated with Nutlin-3, an MDM2 specific inhibitor. As a result of the overexpression of MDM2 gene in LNCaP-MST (10.3-fold), Thrombospondin 1 (THBS1), Tumor necrosis factor alpha (TNF-α) and Matrix metallopeptidase 9 (MMP9) were also found to be significantly up-regulated while genes such as Epiregulin (EREG), Tissue inhibitor of metalloproteinases 1 (TIMP1) were down-regulated. Also, we determined the total MMP activity and MMP9 expression in LNCaP, LNCaP-MST and SJSA-1 cells. Our results indicated that MDM2 level is positively correlated with MMP activity and MMP9 secretion. Our findings offer strong supporting evidence that MDM2 can impact growth and m...Continue Reading

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Citations

Mar 13, 2019·The Journal of Gene Medicine·Fan-Kai XiaoLi-Dong Wang
Aug 15, 2018·Histochemistry and Cell Biology·Dariusz Stępiński
Mar 31, 2019·International Journal of Molecular Sciences·Renée Laufer-AmorimSilvia Regina Rogatto
Aug 24, 2019·Cancer Cell International·Helei HouXiaochun Zhang
Jan 21, 2021·International Journal of Molecular Sciences·Qi LiuWei Zhang

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Methods Mentioned

BETA
ubiquitination
PCR
Assay
protein assay
electrophoresis
transgenic
Profiler

Software Mentioned

RT 2 gene expression Profiler
ImageJ

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