Abstract
Drug-resistant TB poses a major threat to control of TB worldwide. Despite progress in the detection of Multidrug-resistant TB (MDR-TB) cases, a major diagnostic gap remains: 55% of reported TB patients estimated to have MDR-TB were not detected in 2013. MDR-TB antigens were conjugated to CNBr-activated Sepharose 4B. Specific polyclonal antibodies against MDR-TB Ags were prepared in rabbits using two boosted injections of the MDR-TB antigen. The antibodies were purified and treated with susceptible TB to remove any non-specific and cross-reactive antibodies. In the present study, comparative analysis of electrophoretic pattern of different antigens of INH/RIF-resistant TB were studied for identifying protein profiles. A RIF-resistant TB antigen was shown here to have different protein profiles from INH-resistant TB isolate. The results of Western blotting analysis showed that in the RIF- and INH-resistant antigenic fractions some bands of 14.4 and 45 kDa as immunogenic were common. Moreover, four bands of RIF-resistant TB antigen fractions (16, 19, 21, and 45 KDa) and one band of INH-resistant TB (about 26 KDa) were detected as diagnostic antigens. This study suggests that the Western blot is an accurate test to survey INH- and...Continue Reading
References
Dec 1, 1977·Infection and Immunity·M HarboeN H Axelsen
May 7, 1976·Analytical Biochemistry·M M Bradford
Jul 1, 1989·The Journal of Clinical Investigation·R S WallisJ J Ellner
Aug 15, 1970·Nature·U K Laemmli
Mar 1, 1994·Clinical and Diagnostic Laboratory Immunology·C CoetsierC Cocito
Jul 1, 1996·Antimicrobial Agents and Chemotherapy·T R GarbeV Deretic
Jun 18, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·S T BeckA W Ferreira
May 19, 2006·Expert Review of Molecular Diagnostics·Madhukar PaiKeertan Dheda
Jan 18, 2007·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Xin JiangHonghai Wang
Dec 18, 2007·Clinical Biochemistry·Samir El-MasryMohamed Khairy Al-Badrawey
Oct 26, 2011·The Journal of Infectious Diseases·Jacqueline M AchkarVictoria O Kasprowicz
Dec 22, 2011·Microbial Cell Factories·Ramsés A Gamboa-SuasnavartMauricio A Trujillo-Roldán
Feb 3, 2012·The European Respiratory Journal·J GrenierM Pai
Nov 8, 2012·PloS One·Eduardo Martins de SousaAna Paula Junqueira-Kipnis
Jan 15, 2014·BMC Infectious Diseases·Vanina MeyssonnierJérôme Robert
Jan 1, 2013·The Open Microbiology Journal·Mohammad VarahramAli Akbar Velayati