Measles virus glycoprotein-pseudotyped lentiviral vectors are highly superior to vesicular stomatitis virus G pseudotypes for genetic modification of monocyte-derived dendritic cells.

Journal of Virology
J-M HumbertF Halary

Abstract

Dendritic cells (DCs) are potent antigen-presenting cells capable of promoting or regulating innate and adaptive immune responses against non-self antigens. To better understand the DC biology or to use them for immune intervention, a tremendous effort has been made to improve gene transfer in these cells. Lentiviral vectors (LVs) have conferred a huge advantage in that they can transduce nondividing cells such as human monocyte-derived DCs (MDDCs) but required high amounts of viral particles and/or accessory proteins such as Vpx or Vpr to achieve sufficient transduction rates. As a consequence, these LVs have been shown to cause dramatic functional modifications, such as the activation or maturation of transduced MDDCs. Taking advantage of new pseudotyped LVs, i.e., with envelope glycoproteins from the measles virus (MV), we demonstrate that MDDCs are transduced very efficiently with these new LVs compared to the classically used vesicular stomatitis virus G-pseudotyped LVs and thus allowed to achieve high transduction rates at relatively low multiplicities of infection. Moreover, in this experimental setting, no activation or maturation markers were upregulated, while MV-LV-transduced cells remained able to mature after an ap...Continue Reading

References

Jul 20, 1995·Nature·B G CocksG Aversa
Apr 1, 1997·Immunology and Cell Biology·G AversaJ E De Vries
Apr 29, 1998·Cancer Immunology, Immunotherapy : CII·E GilboaH K Lyerly
Jun 8, 1999·The Journal of Experimental Medicine·D UnutmazD R Littman
Jun 3, 2000·Annual Review of Immunology·J BanchereauK Palucka
Sep 6, 2000·Nature·H TatsuoY Yanagi
Feb 24, 2001·The Journal of Experimental Medicine·M V DhodapkarN Bhardwaj
Aug 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M KruseA Steinkasserer
Mar 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Miguel RellosoAngel L Corbí
Jun 7, 2002·Journal of Virology·Carmen P AlvarezRafael Delgado
Jul 12, 2002·Current Gene Therapy·Antoni RibasJames S Economou
Aug 31, 2002·Virology·Yusuke YanagiHiroko Minagawa
Jan 15, 2003·Journal of Leukocyte Biology·Ian D DavisJonathan Cebon
Mar 5, 2003·Annual Review of Immunology·Ralph M SteinmanMichel C Nussenzweig
Jun 5, 2003·The Journal of Clinical Investigation·Christoph EsslingerH Robson MacDonald
Oct 7, 2004·The Journal of Gene Medicine·Karine BreckpotKris Thielemans
Nov 17, 2004·The Journal of Experimental Medicine·Jean-François ArrighiVincent Piguet
Nov 30, 2004·Nature Medicine·Wei LuJean-Marie Andrieu
Apr 2, 2005·Nature Reviews. Immunology·Jacques Banchereau, A Karolina Palucka
Jan 26, 2006·Journal of Leukocyte Biology·Felix I L ClanchyJohn A Hamilton
Mar 16, 2006·Journal of Virology·Lot de WitteTeunis B H Geijtenbeek

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Aug 25, 2015·Current Opinion in Pharmacology·Camille LévyFrançois-Loïc Cosset
May 7, 2011·Journal of Experimental & Clinical Cancer Research : CR·Mohamed T Abdel azizFatma M Taha
Jul 23, 2016·Frontiers in Immunology·Thomas D Norton, Elizabeth A Miller
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