Measles virus hemagglutinin protein expressed in transgenic lettuce induces neutralising antibodies in mice following mucosal vaccination

Vaccine
Diane E WebsterSteve L Wesselingh

Abstract

Plant-made oral vaccines have the potential to overcome many of the limitations of traditional vaccines. Here we report on progress towards a lettuce-made measles vaccine. Lettuce is a palatable species which exhibits rapid growth in contained hydroponic systems and produces negligible quantities of toxins. Measles virus hemagglutinin (MV-H) protein was successfully expressed in transgenic lettuce and found to be immunogenic in mice. Lettuce extracts containing MV-H protein induced MV neutralising antibodies following intraperitoneal injection and intranasal inoculation of mice. Using a sequential prime-boost strategy in which mice were vaccinated with MV-H DNA followed by an orally delivered freeze-dried MV-H lettuce formulation a 10-fold increased in MV-specific IgG titers was observed relative to mice vaccinated with control lettuce formulations (p=0.05). MV-H protein was stable in freeze-dried lettuce for up to 13 months at room temperature, and survived at least a week at temperatures as high as 50 degrees C. This research represents a significant step towards the development of measles vaccine formulation that is effective, temperature-stable, easy to administer in a resource-poor setting and amenable to large scale manuf...Continue Reading

Citations

Jan 19, 2011·Plant Cell Reports·Claire A PenneyAmanda M Walmsley
Aug 3, 2010·Expert Review of Vaccines·Matthew Paul, Julian K-C Ma
Oct 11, 2008·Expert Review of Vaccines·Rory D de VriesRik L de Swart
Oct 22, 2008·Critical Reviews in Biotechnology·Pervin Basaran, Emilio Rodríguez-Cerezo
Mar 12, 2008·Journal of Cellular Physiology·Chiara LicoLuca Santi
Mar 18, 2010·Plant Biotechnology Journal·Edward P Rybicki
Aug 8, 2007·International Journal for Parasitology·Lina WangRoss L Coppel
Jun 13, 2012·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Kayo YoshimatsuMasahiro Nishijima

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