Measurement of α-dicarbonyl compounds in human saliva by pre-column derivatization HPLC.

Clinical Chemistry and Laboratory Medicine : CCLM
Xin-Jie WangWen-Yun Gao

Abstract

Background α-Dicarbonyl compounds (α-DCs) have been detected in body fluids including plasma and urine and elevation of this sort of compounds in vivo has been associated with the development of many kinds of chronic diseases. However whether α-DCs are present in human saliva, and if their presence/absence can be related with various chronic diseases is yet to be determined. Methods In this study, a pre-column derivatization HPLC-UV method was developed to measure 3-deoxyglucosone (3-DG), glyoxal (GO), methylglyoxal (MGO), diacetyl (DA), and pentane-2,3-dione (PD) in human saliva employing 4-(2,3-dimethyl-6-quinoxalinyl)-1,2-benzenediamine (DQB) as a derivatizing reagent. The derivatization of the α-DCs is fast and the conditions are facile. The method was evaluated and the results show that it is suitable for the quantification of α-DCs in human saliva. Results In the measurements of these α-DCs in the saliva of 15 healthy subjects and 23 type 2 diabetes mellitus (T2DM) patients, we found that the concentrations of GO and MGO in the saliva of the diabetic patients were significantly higher than those in healthy subjects. As far as we know, this is the first time that salivary α-DC concentrations have been determined and associ...Continue Reading

References

Jul 1, 1991·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·A M DarwazehP J Lamey
Sep 24, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·T Niwa
Jul 5, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Eliaz Kaufman, Ira B Lamster
Nov 12, 2002·The Journal of Prosthetic Dentistry·Ana M Diaz-Arnold, Cindy A Marek
Jan 1, 1960·Scandinavian Journal of Clinical and Laboratory Investigation·E RAABO, T C TERKILDSEN
Jul 23, 2005·Annals of the New York Academy of Sciences·Anke FörsterThomas Henle
Jan 9, 2007·Chemico-biological Interactions·Nandita ShangariPeter J O'brien
Jun 13, 2008·American Journal of Nephrology·Keisuke NakayamaSadayoshi Ito
Aug 29, 2009·Journal of Agricultural and Food Chemistry·Jenny Gobert, Marcus A Glomb
Oct 16, 2009·Oral Oncology·Rafael M Nagler
Nov 26, 2010·Dental Clinics of North America·Daniel Malamud
Jul 5, 2011·Analytical and Bioanalytical Chemistry·Stefan MittelmaierMonika Pischetsrieder
Aug 29, 2014·The Journal of Biological Chemistry·Christian HenningMarcus A Glomb
Oct 9, 2014·Journal of Diabetes Science and Technology·Shruti GuptaDeepti Sharma
Dec 3, 2014·Respiratory Physiology & Neurobiology·Katia AvezovDror Aizenbud
Feb 18, 2016·BMC Research Notes·Monica Virginia Viegas Lima-AragãoRosane Nassar Meireles Guerra
Jul 19, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yuki OgasawaraMakoto Arai
May 6, 2017·International Journal of Molecular Sciences·Sebastian BringsPeter P Nawroth
Jan 15, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xin-Jie WangWen-Yun Gao

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Citations

Aug 26, 2020·Journal of the American Society for Mass Spectrometry·Jingwen HuHuwei Liu

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