Measurement of 2,6-diamino-4-hydroxy-5-formamidopyrimidine and 8-oxo-7,8-dihydroguanine in isolated DNA exposed to gamma radiation in aqueous solution

Carcinogenesis
Thierry DoukiJean Cadet

Abstract

Hydroxyl radical-mediated modifications of nucleobases are among the main deleterious processes induced in DNA by ionizing radiation. Determination of the underlying mechanisms in both isolated and cellular DNA requires the development of accurate assays for the detection of modified bases. High-performance liquid chromatography associated with electrochemical detection (HPLC-EC) and gas chromatography coupled to mass spectrometry (GC-MS) are the two main methods used for this purpose. In the present work, HPLC-EC was applied to the measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) following enzymatic digestion of DNA. Evidence was provided for the quantitative aspect of the latter step. Moreover, a GC-MS assay was designed for the detection of 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyGua). It was first shown that the widely used hot formic acid hydrolysis of DNA induced a complete decomposition of the lesion. A similar observation was also made for 4,6-diamino-5-formamidopyrimidine (FapyAde). A milder DNA hydrolyzing agent, HF in pyridine, was substituted to HCOOH for the quantitative release of FapyGua from DNA. The radiation-induced formation of 8-oxodGuo and FapyGua in aqueous solution of DNA was compar...Continue Reading

Citations

Feb 2, 2002·Chemistry : a European Journal·Lars T Burgdorf, Thomas Carell
May 11, 2006·International Archives of Occupational and Environmental Health·A Pilger, H W Rüdiger
Mar 5, 1999·Mutation Research·J CadetS Sauvaigo
Aug 6, 2002·Free Radical Biology & Medicine·Jean CadetJean-Luc Ravanat
Feb 3, 2000·Free Radical Biology & Medicine·M A GraziewiczB Tudek
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Fu-Chou ChengTung-Hu Tsai
Jan 14, 2004·Journal of Toxicology and Environmental Health. Part a·Gülnur AndicanGülden Burçak
Sep 11, 2008·Journal of the Royal Society, Interface·Supat Jiranusornkul, Charles A Laughton
Mar 6, 2003·The Journal of Clinical Investigation·Lawrence J MarnettJames D West
Feb 26, 2005·Organic & Biomolecular Chemistry·Anne LyJamie R Milligan
Oct 19, 2002·Critical Reviews in Clinical Laboratory Sciences·Gunther GuetensErnst A de Bruijn
Sep 16, 2011·Free Radical Research·Jean-Luc Ravanat
Mar 31, 1999·Free Radical Research·J CadetS Sauvaigo
Mar 21, 2006·International Journal of Radiation Biology·Thierry DoukiJean Cadet
Jul 19, 2006·International Journal of Radiation Biology·Anne LyJamie R Milligan
Mar 17, 2010·Free Radical Biology & Medicine·Jean Cadet, Henrik Poulsen
Apr 7, 2010·Free Radical Biology & Medicine·Jean CadetJean-Luc Ravanat
Apr 9, 2015·Chemical Research in Toxicology·Aaron M FlemingCynthia J Burrows
Oct 18, 2008·Free Radical Biology & Medicine·Pawel JarugaMiral Dizdaroglu
Feb 20, 2009·Angewandte Chemie·Chryssostomos ChatgilialogluQuinto G Mulazzani
Jun 10, 2009·Bioorganic & Medicinal Chemistry Letters·Aleksandra A KuznetsovaOlga S Fedorova
Feb 19, 2011·Mutation Research·Jean CadetJean-Luc Ravanat
Jan 11, 2011·Mutation Research·Thomas B KrystonAlexandros G Georgakilas
Jul 21, 2016·International Journal of Radiation Biology·Mhamad Abou-HamdanChristian Triantaphylides
Nov 21, 2008·Dose-response : a Publication of International Hormesis Society·Dmytro GrygoryevJohn D Zimbrick
Jun 10, 2010·European Journal of Mass Spectrometry·Herbert Oberacher
May 17, 2001·The Journal of Biological Chemistry·M E BranumA Sancar
Nov 21, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jens Sobek, Ralph Schlapbach
Aug 1, 2008·Accounts of Chemical Research·Jean CadetJean-Luc Ravanat
Dec 22, 2005·Journal of the American Chemical Society·Matthias OberThomas Carell

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