PMID: 7025068Apr 1, 1981Paper

Measurement of 6-keto-prostaglandin E1 alpha in human plasma with radioimmunoassay: effect of prostacyclin infusion

Prostaglandins and Medicine
D Ylikorkala, L Viinikka

Abstract

A radioimmunoassay for 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha), a stable hydration product of prostacyclin (PGI2), is described. The method utilises plasma extraction with ethyl acetate and saturation analysis. Antiserum was produced in rabbits against 6-keto-PGF1 alpha-BSA conjugate, and its cross-reactions with 22 prostaglandins and related compounds were insignificant. The mean mass of added 6-keto-PGF1 alpha required to displace zero-point binding by 50 % was 55.6 +/- 5.1 pg (mean +/- SD, N = 10). The method blank was 7.8 +/- 2.4 pg/ml (N = 10), resulting in a detection limit of 12.6 pg/ml (mean blank + 2 SD). The intra-assay variation was between 4.1 - 8.2 % at concentrations between 150.3 pg/ml and 560.7 pg/ml (N = 10). The interassay variation was 12.4 % (N = 15). The recoveries of 100 pg/ml or 200 pg/ml added to the plasma samples (N = 10) were 90.2 % and 95.4 %, respectively. The 6-keto-PGF1 alpha concentrations in healthy subjects ranged from 92.7 to 394.5 pg/ml with a mean of 182.5 pg/ml in plasma. The presence of acetylsalicylic acid or indomethacin in the collection tubes caused no changes in plasma 6-keto-PGF1 alpha concentration. Intravenous infusion of PGI2 (1-8 ng/kg/min) into 6 healthy women caused l...Continue Reading

References

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May 17, 1979·The New England Journal of Medicine·S Moncada, J R Vane
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Citations

May 1, 1984·Prostaglandins·B Rosenkranz, J C Frölich
Mar 1, 1990·Prostaglandins·A Aitokallio-Tallberg
Nov 1, 1982·Prostaglandins, Leukotrienes, and Medicine·O YlikorkalaL Viinikka
Mar 15, 2002·Early Human Development·Patrik LassusSture Andersson
Aug 1, 1986·Archives of Disease in Childhood·O YlikorkalaL Rovamo
Aug 1, 1986·American Journal of Obstetrics and Gynecology·O YlikorkalaL Viinikka
Apr 15, 1985·American Journal of Obstetrics and Gynecology·P JouppilaO Ylikorkala
Mar 1, 1985·American Journal of Obstetrics and Gynecology·P JouppilaO Ylikorkala
Sep 1, 1986·British Journal of Obstetrics and Gynaecology·O YlikorkalaL Viinikka
May 1, 1982·British Journal of Obstetrics and Gynaecology·L Mäkäräinen, O Ylikorkala
Oct 1, 1981·British Journal of Obstetrics and Gynaecology·O YlikorkalaL Viinikka
Apr 1, 1982·The American Journal of Cardiology·G G SerneriL Scarti
Jun 29, 2021·Animal Models and Experimental Medicine·Rune B StrandbyLars-Bo Svendsen
Mar 1, 1987·Human Pathology·G A FitzgeraldJ A Oates

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