Measurement of 8-iso-prostaglandin F2alpha in biological fluids as a measure of lipid peroxidation

Methods in Molecular Biology
Stefania TacconelliPaola Patrignani

Abstract

Several lines of evidence suggest that reactive oxygen species are implicated in human disease, including atherosclerosis, hypertension, and restenosis after angioplasty. The measurement of F(2)-isoprostanes (F(2)-iPs), formed nonenzymatically through free radical catalyzed attack on esterified arachidonate, provides a reliable tool for identifying populations with enhanced rates of lipid peroxidation. Among F(2)-isoPs, 8-iso-PGF(2alpha) (also referred to IPF(2alpha)-III) and IPF(2alpha)-VI are the most frequently measured in biological fluids. A variety of methods have been proposed to measure F(2)-isoprostanes in urine and plasma. Mass spectrometry has been developed for the measurement of both F(2)-isoprostanes but its use is limited as it is time-consuming and highly expensive. We have developed validated enzyme immunoassay (EIA) and radioimmunoassay (RIA) techniques using highly specific antisera for the measurement of 8-iso-PGF(2alpha). In contrast, the commercially available immunoassay kits are limited for their poor specificity. The measurement of specific isoprostanes, such as 8-iso-PGF(2alpha), in urine is a reliable, noninvasive index of lipid peroxidation that is of valuable help in dose-finding studies of natural ...Continue Reading

Citations

May 24, 2014·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Linsey E HaswellFrazer J Lowe
Aug 9, 2013·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Jung Hyun KwakJong Ho Lee
Aug 4, 2018·Analytical and Bioanalytical Chemistry·Zhi-Xin YuanChristopher E Ramsden
Apr 30, 2016·Journal of the American Heart Association·Wenyuan LiMurray A Mittleman
Oct 18, 2014·Journal of Clinical Neurology·Ramin AnsariJin Jun Luo
Oct 15, 2013·Basic & Clinical Pharmacology & Toxicology·Melania DovizioPaola Patrignani
Nov 27, 2017·Metabolomics : Official Journal of the Metabolomic Society·Junjie ZhangChun Cai
Jan 11, 2019·International Journal of Molecular Sciences·Rita Moretti, Paola Caruso

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