Measurement of amphotericin B concentration by resonant Raman spectroscopy--a novel technique that may be useful for non-invasive monitoring

Medical Mycology
William V La ViaMark S Borchert

Abstract

We wished to determine whether Resonant Raman Spectroscopy (RRS) could be used to measure Amphotericin B (AmB) at therapeutic and subtherapeutic concentrations in a model system mimicking the anterior chamber of the eye. The goal was to develop a technique for non-invasive measurement of AmB levels in the aqueous humor (AH) of the eye. A krypton-ion laser source (406.7 nm) was used for excitation and Resonant Raman Spectra were captured with a confocal system in an anterior chamber (AC) model. These spectra were used to develop a correlation curve for prediction of AmB levels. Subsequently, one rabbit was evaluated with this system after 5 days of intravenous AmB administration (1 mg/kg/day) and AmB concentrations measured by RRS were compared to those measured by high-performance liquid chromatography (HPLC). AmB exhibited a unique spectral peak at 1557 cm(-1). Integrated area of this peak linearly correlated with AmB concentration in our model AC. When integrated peak area from multiple in vivo measurements in one animal at steady-state was plotted on this correlation curve, we were able to predict AmB levels. These closely approximated those measured by HPLC. These measurements were not significantly affected by photobleachi...Continue Reading

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Citations

Jul 20, 2011·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Mari ElshoutJan S Schouten
Jul 7, 2007·Journal of Biomedical Optics·Theoharia SideroudiGeorge A Voyiatzis
Apr 5, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Mariusz GagośKrzysztof Kostro
Feb 29, 2008·Journal of Pharmaceutical Sciences·Cushla M McGoverinKeith C Gordon
Jun 1, 2006·Lasers in Surgery and Medicine·Theoharia I SideroudiGeorge A Voyiatzis
Apr 13, 2011·The Journal of Physical Chemistry. B·Mariusz GagośWiesław I Gruszecki
Aug 27, 2021·Advanced Drug Delivery Reviews·Benjamin A KuzmaConor L Evans

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