Measurement of anti-beta amyloid antibodies in human blood

Journal of Neuroimmunology
Paul SzaboNorman Relkin

Abstract

The human IgG repertoire contains endogenous antibodies against beta amyloid (Aβ) that may be relevant to the pathogenesis and treatment of Alzheimer's disease. There have been widely disparate estimates of the levels of these antibodies in human plasma. We identify factors that have contributed to these disparities and describe improved methods for measuring anti-Aβ antibodies in blood. These methods include isolating immunoglobulin by thiophilic chromatography and using chaotropic salts to dislodge weakly bound antibodies without significantly reducing the binding of specific anti-Aβ antibodies. Using these methods, we show that human blood contains polyvalent IgG antibodies that bind to Aβ with relatively low avidity and specificity, as well as IgG antibodies that bind to linear and conformational epitopes on amyloid monomers and aggregates with moderate to high avidity.

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Citations

Apr 25, 2014·Journal of Clinical Immunology·Norman Relkin
Dec 30, 2014·Clinical and Experimental Immunology·N Relkin
Feb 18, 2016·Neural Plasticity·Jaume FolchAntoni Camins
Apr 30, 2015·Analytical Biochemistry·Amanda C CrisostomoJohn M Finke
Mar 13, 2014·Journal of Internal Medicine·L LannfeltE R Siemers
Sep 25, 2015·Journal of Alzheimer's Disease : JAD·Sofia SöllvanderDag Sehlin
Sep 4, 2015·International Immunopharmacology·David A LoefflerMary P Coffey
May 16, 2015·Neurología : publicación oficial de la Sociedad Española de Neurología·J FolchA Camins
May 15, 2013·International Immunopharmacology·Lynnae M SmithDavid A Loeffler
May 25, 2016·Neurology. Neuroimmunology and Neuroinflammation·Elysse M KnightJohn W Steele
Oct 28, 2017·Sensors·Goulielmos-Zois GaryfallouEileen Hao Yu
Jan 1, 2011·Journal of Central Nervous System Disease·Rakez KayedAlan D T Barrett
Feb 15, 2019·Laboratory Investigation; a Journal of Technical Methods and Pathology·Rudy J CastellaniGeorge Perry
Apr 13, 2013·The Journal of Biological Chemistry·Abhay P SagareBerislav V Zlokovic
Apr 7, 2017·Neurology·Norman R RelkinUNKNOWN Alzheimer's Disease Cooperative Study
Apr 4, 2021·International Journal of Molecular Sciences·Stefano SorrentinoGiuseppe Di Fede

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