PMID: 9534209Apr 16, 1998Paper

Measurement of anti-dsDNA: a comparative study of two ELISA and the Crithidia assay

Pathology
K H WongC S Lau

Abstract

We compared the measurement of anti-dsDNA by a commercial ELISA test (DIASTAT), an in-house ELISA and the Crithidia luciliae assay in cross-sectional sera samples of 209 systemic lupus erythematosus (SLE) patients and 64 patients with a variety of rheumatological, autoimmune and non-autoimmune diseases in Hong Kong. The Crithidia assay was found to be the least sensitive (17%) but most specific (95%) method for detection of a positive result in SLE patients. The DIASTAT assay has a higher sensitivity (68%) but lower specificity (80%) than the in-house ELISA test (32% sensitivity and 89% specificity). The positive predictive value of the three assays are comparable at 90-92% while DIASTAT had the highest negative predictive value (44%). There was good linear correlation (r = 0.7) between the two ELISAs. ELISA can serve as a useful screening test for anti-dsDNA in SLE patients and doubtful cases can then be confirmed by another method such as radio-immunoassay.

Citations

Oct 17, 2002·Arthritis and Rheumatism·Arthur F KavanaughUNKNOWN American College of Rheumatology Ad Hoc Committee on Immunologic Testing Guidelines
Mar 16, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Kyeong Hee KimWon Tae Chung
Aug 11, 2006·Lupus·A M Rouquette, C Desgruelles

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