PMID: 9005954Jan 15, 1997Paper

Measurement of antibody/antigen association rate constants in solution by a method based on the enzyme-linked immunosorbent assay

Journal of Immunological Methods
F HardyM E Goldberg

Abstract

A reliable ELISA based method has been developed for measuring in solution antigen/antibody association rate constants. Its rationale is as follows: antigen and antibody are mixed in solution to initiate the association. At different time intervals aliquots are withdrawn to determine by an indirect ELISA the amount of free antibody that remains in solution. The disappearance of the free mAb reflects the time course of the association reaction. To test the validity of this method, the association rate constant of a monoclonal antibody for its antigen was measured and compared with that obtained previously by a method using fluorescence. The good agreement between the results obtained with the ELISA-based method and those obtained previously by fluorescence measurement indicates that the method described permits determination of true association rate constants in solution. The present method offers several advantages. It uses only minute amounts of sample which need not be purified; it requires no radioactive or fluorescent labelling of the antibody or the antigen, and it can be applied to any type of complex between macromolecules if an ELISA test can be set up to detect quantitatively one of the macromolecules.

References

Apr 1, 1993·Current Opinion in Immunology·M E Goldberg, L Djavadi-Ohaniance

❮ Previous
Next ❯

Citations

Jan 22, 2003·Biochemical and Biophysical Research Communications·Ling DongMelitta Schachner
Oct 3, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·R R GolgherS E Grossberg
Sep 21, 2013·International Journal of Molecular Sciences·Jianhao WangPengju Jiang
Jan 29, 2014·International Journal of Molecular Sciences·Lin QiuPengju Jiang
Oct 8, 2008·Biosensors & Bioelectronics·Chia-Hsien YehYu-Cheng Lin
Apr 11, 2000·Connective Tissue Research·R SrinivasanA George
Apr 10, 2008·Journal of Bioscience and Bioengineering·Dong Hwan ChoiSuteaki Shioya
Nov 2, 2016·Analytical Chemistry·Barrett J NehillaJames J Lai
Jul 9, 2010·Journal of Leukocyte Biology·Hiromichi YamashiroNaohiro Seo
Jun 1, 2007·The Journal of Biological Chemistry·Nuria Jiménez-JuárezAlejandra Bravo
Mar 11, 2018·Biochemistry. Biokhimii︠a︡·D V SotnikovB B Dzantiev
May 17, 2013·The Journal of Biological Chemistry·Sonia MediouniErwann P Loret
Nov 3, 2017·Cold Spring Harbor Protocols·Frances Weis-Garcia, Robert H Carnahan
Jul 12, 2018·European Biophysics Journal : EBJ·Emilie Vanhove, Marc Vanhove
Oct 16, 1999·European Journal of Biochemistry·M AtgerE Milgrom
Nov 23, 2006·Journal of Agricultural and Food Chemistry·Tiejun LiFengquan Liu
May 1, 2007·Protein Expression and Purification·Ravinder KaurRaj Bhatnagar
Jun 29, 1999·Analytical Chemistry·D S Hage

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.