Measurement of bone blood flow using the hydrogen washout Technique-Part I: quantitative evaluation of tissue perfusion in the laboratory rat

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Michael PelzerAllen T Bishop

Abstract

The measurement of blood flow in bone is of interest in both clinical and experimental settings. Such determinations would ideally provide accurate, quantitative, and reproducible data with relatively simple and safe technology, even in the small bones of experimental animals. Methods that provide absolute or quantitative measurements include positron emission tomography, "isotope fractioning" using radioactive or fluorescent-labeled microspheres, and measurement of the rate of washout of diffusible tracers delivered either by injection or inhalation. In this article, we describe in detail a modification of the original Whiteside hydrogen washout technique, using modern hydrogen sensors, a micromanipulator for probe placement, and custom software that greatly simplifies blood flow determination and is effective in the small bones of experimental animals.

References

Jan 1, 1980·Calcified Tissue International·P Tothill, J M MacPherson
Jul 1, 1993·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·G S LaustenB Dahl
Mar 20, 2002·The Journal of Hand Surgery·Saichol WongtrakulPatricia F Friedrich
Feb 1, 1964·Circulation Research·K AUKLANDR W BERLINER
Jan 30, 2004·Microsurgery·Osami SuzukiPatricia F Friedrich

❮ Previous
Next ❯

Citations

Jan 27, 2011·The Journal of Bone and Joint Surgery. American Volume·Mikko LarsenAllen T Bishop
May 14, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Michael PelzerAllen T Bishop
Nov 22, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Goetz A GiesslerAllen T Bishop
Apr 3, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Wouter F WillemsAllen T Bishop
Sep 16, 2010·Microsurgery·Mikko LarsenAllen T Bishop
Nov 30, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Tiago MattarAllen T Bishop
Mar 30, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Wouter F WillemsAllen T Bishop
Apr 6, 2016·Acta Biomaterialia·Daoli ZhaoWilliam R Heineman
Oct 17, 2012·Acta Biomaterialia·Julia KuhlmannFrank Witte
Feb 6, 2019·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Khaled Q Al HamadNadim Z Baba
Jan 8, 2013·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Noor A NawaflehMuhanad M Hatamleh
Jul 6, 2014·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Evanthia AnadiotiFang Qian
Apr 26, 2020·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Elie E Daou, Nadim Z Baba
Jun 23, 2012·Clinical Orthopaedics and Related Research·Wouter F WillemsAllen T Bishop

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.