PMID: 7541455Jan 1, 1995Paper

Measurement of c-erbB-2 proteins in sera from patients with carcinomas and in breast tumor tissue cytosols: correlation with serum tumor markers and membrane-bound oncoprotein

Journal of Clinical Laboratory Analysis
J T WuL Bryson

Abstract

Using a commercial kit with antibodies against the ectodomain of c-erbB-2 protein, we detected c-erbB-2 immunoreactivity in human serum. We found that the percentages of patients with elevated serum c-erbB-2 immunoreactivities were 35, 21, and 9% in breast, prostate, and ovarian carcinoma, respectively. The majority of the elevated immunoreactivities were associated with sera containing highly elevated tumor markers with the highest in breast carcinoma (35%) and lowest in ovarian cancer (9%). Excellent correlations were also observed between the serum levels of c-erbB-2 immunoreactivity and the dominant tumor markers in serial specimens from individual cancer patients. We could also detect the c-erbB-2 immunoreactivity in the cytosols prepared from the breast tumor tissue for estrogen and progesterone receptor (ER & PgR) measurements using the same commercial kit for serum studies, and the intact c-erbB-2 oncoprotein (p185) in the extracts of the tissue membrane fractions with a different kit designed for tissue extract. The level of c-erbB-2 immunoreactivity in the cytosol from 124 human breast tumor specimens had an excellent correlation with the cell membrane concentrations of p185 (gamma = 0.89). Most of the elevated cytoso...Continue Reading

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Citations

Jan 1, 1995·Journal of Clinical Laboratory Analysis·J T WuR Stephenson
May 20, 2005·Virchows Archiv : an International Journal of Pathology·Chun-Mei ChengYasuo Takano
Feb 23, 2008·Biochimica Et Biophysica Acta·Jacqueline M LafkyNita J Maihle
Mar 4, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Alexandra F LearyMitch Dowsett
Aug 1, 1997·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·S MielkeW Kuhn
Oct 22, 2015·American Journal of Clinical Pathology·Daniela FurrerCaroline Diorio
May 4, 2013·Cancer Prevention Research·William B ArmstrongFrank L Meyskens
Sep 19, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shahrokh F ShariatPierre I Karakiewicz
Jul 13, 2004·Clinical Breast Cancer·Walter P CarneyChristopher P Price

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