Measurement of caspase activity in individual cells reveals differences in the kinetics of caspase activation between cells

Cell Death and Differentiation
M J Morgan, A Thorburn

Abstract

Most forms of apoptosis involve activation of caspases and it is likely that differences between cells in their ability to activate caspases contributes to the responsiveness of any given cell within a population to apoptotic stimuli. To study the molecular mechanisms that underlie such differences, it is necessary to measure caspase activity in individual cells. Here, we describe a method that allows the continuous monitoring of caspase activity in individual, living mammalian cells. This approach allows studies of the kinetics of caspase activation to be performed in individual cells within a population. We demonstrate that in a group of cells where some cells die and some cells survive in response to the same stimulus, the cells that die can be differentiated from those that survive based on the amount of caspase activity in each cell several hours before death occurs.

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Citations

Nov 26, 2003·Cellular Signalling·Andrew Thorburn
Oct 29, 2003·Journal of Immunological Methods·Naomi ZurgilMordechai Deutsch
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Young-wook JunA Paul Alivisatos
Jan 17, 2003·Molecular Biology of the Cell·Jacqueline ThorburnAndrew Thorburn
Feb 24, 2009·Biosensors & Bioelectronics·Ri Mi LeeKyung-Hwa Yoo
Apr 16, 2014·Scientific Reports·Eva BrauchleKatja Schenke-Layland
Sep 17, 2004·Biochemical and Biophysical Research Communications·Bin Zhang
Nov 10, 2015·Chemical Reviews·Marcin PorebaMarcin Drag
May 27, 2004·The American Journal of Pathology·Liusheng HePeter E Lipsky
Aug 2, 2005·Biophysical Journal·Tobias KohlPetra Schwille
May 18, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Pravin K NaoghareJoon Myong Song
Sep 30, 2010·The Analyst·Katharina WelserJonathan W Aylott
Nov 25, 2003·Diabetes·Martin KöhlerPer-Olof Berggren
Nov 5, 2005·Journal of Advanced Nursing·Jeanette Hewitt, Michael Coffey
May 26, 2009·International Journal of Offender Therapy and Comparative Criminology·Andrew Day, Tony Ward
Oct 15, 2010·Bioscience Reports·Dave BoucherJean-Bernard Denault

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