Measurement of Cell-Matrix Adhesion at Single-Cell Resolution for Revealing the Functions of Biomaterials for Adherent Cell Culture

Analytical Chemistry
Sifeng MaoJin-Ming Lin

Abstract

Cell adhesion is essential for a cell to maintain its functions, and biomaterials acting as the extracellular matrix (ECM) play a vital role. However, conventional methods for evaluating the functions of biomaterials become insufficient and sometimes incorrect when we give a deeper insight into single-cell research. In this work, we reported a novel methodology for the measurement of cell-matrix adhesion at single-cell resolution that could precisely evaluate the functions of biomaterials for adherent cell culture. A microfludic device, a live single-cell extractor (LSCE), was used for cell extraction. We applied this method to evaluate various modified biomaterials. The results indicated that poly(l-polylysine) (PLL)-coated glass and fibronection (FN)-coated glass slides showed the best biocompatibility for adherent cell culture following by the (3-aminopropyl)triethoxysilane (APTES)-coated glass, while piranha solution treated glass slide and octadecyltrichlorosilane (OTS)-coated glass showed weak biocompatibilities. Furthermore, APTES, PLL, and FN modifications enhanced the cell heterogeneity, while the OTS modification weakened the cell heterogeneity compare to the initial piranha solution treated glass. The method not only...Continue Reading

References

May 4, 2010·Annual Review of Biomedical Engineering·Richard N Zare, Samuel Kim
Jul 20, 2010·Biochemical and Biophysical Research Communications·Catriona KellyNeville H McClenaghan
Oct 29, 2011·Trends in Cell Biology·Dongeun HuhDonald E Ingber
Jul 17, 2012·Bioanalysis·Xiujun James LiZhihong Nie
Mar 7, 2014·Nature Communications·Aniruddh SarkarJongyoon Han
Jul 24, 2015·Experimental Biology and Medicine·Lawrence A VernettiD Lansing Taylor
Jul 28, 2015·Advanced Drug Delivery Reviews·Yosuke K Kurokawa, Steven C George
Feb 24, 2016·Analytical Chemistry·Amy MongersunPaul Abbyad
Jun 24, 2016·Advanced Materials·Nathalie Brandenberg, Matthias P Lutolf
Dec 3, 2016·Angewandte Chemie·Laith F KademChristine Selhuber-Unkel
Dec 9, 2016·Nature Protocols·Rapolas ZilionisLinas Mazutis
Jun 1, 2017·Analytical Chemistry·Maria Elena GallinaGuillem Pratx
Jan 20, 2018·Nature Communications·Davide RissoJean-Philippe Vert
Jan 26, 2018·Nature Communications·Stephen Smith, Ramon Grima
Feb 3, 2018·Chemical Communications : Chem Comm·Qiushi HuangJin-Ming Lin
Dec 11, 2017·ACS Biomaterials Science & Engineering·Yoonmi HongPilnam Kim

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

Progrès en urologie : journal de l'Association française d'urologie et de la Société française d'urologie
Jean-Louis ParientePierre Conort
© 2022 Meta ULC. All rights reserved