Measurement of Cellular Immune Response to Viral Infection and Vaccination.

Frontiers in Immunology
W. BouwmanAnja van de Stolpe

Abstract

Combined cellular and humoral host immune response determine the clinical course of a viral infection and effectiveness of vaccination, but currently the cellular immune response cannot be measured on simple blood samples. As functional activity of immune cells is determined by coordinated activity of signaling pathways, we developed mRNA-based JAK-STAT signaling pathway activity assays to quantitatively measure the cellular immune response on Affymetrix expression microarray data of various types of blood samples from virally infected patients (influenza, RSV, dengue, yellow fever, rotavirus) or vaccinated individuals, and to determine vaccine immunogenicity. JAK-STAT1/2 pathway activity was increased in blood samples of patients with viral, but not bacterial, infection and was higher in influenza compared to RSV-infected patients, reflecting known differences in immunogenicity. High JAK-STAT3 pathway activity was associated with more severe RSV infection. In contrast to inactivated influenza virus vaccine, live yellow fever vaccine did induce JAK-STAT1/2 pathway activity in blood samples, indicating superior immunogenicity. Normal (healthy) JAK-STAT1/2 pathway activity was established, enabling assay interpretation without th...Continue Reading

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Citations

Oct 31, 2020·Cancers·Kirsten Canté-BarrettJules P P Meijerink
Nov 28, 2020·Postgraduate Medicine·Sairaj SatarkerMadhavan Nampoothiri

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Datasets Mentioned

BETA
GSE38351
GSE69606
GSE43777
GSE84331
GSE34205
GSE119322
GSE13486
GSE51997
GSE29614
GSE102459

Methods Mentioned

BETA
biopsies

Software Mentioned

AffyRNAdeg
Affymetrix
statannot
affyQCReport package
Affymetrix package
R
Python

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