Measurement of cerebral glucose utilization from brain uptake of [14C]2-deoxyglucose and [3H]3-O-methylglucose in the mouse

Journal of Pharmacological Methods
K ItoM Hanano

Abstract

Glucose utilization (GU) in the mouse brain in vivo was measured by the simultaneous use of [14C]2-deoxyglucose (2DG), the glucose analogue that can be phosphorylated in the brain, and [3H]3-O-methylglucose (3MG), the nonmetabolizable glucose analogue. Originally, this method was developed by Gjedde et al. (1985) in the rat and in humans. The present study examined the validity of this method in the mouse brain. The effects of urethane and pentobarbital (PB) on GU were also studied. Whereas the distribution volume of 3MG reached a constant value under each condition after 10 min of the tracer circulation, the apparent volume of distribution of 2DG, which increased with time in the awake mice, did not increase so greatly in the anesthetized and hypothermic mice, indicating that the net rate of 2DG phosphorylation is lowered under these conditions. These data were fitted for the conventional three-compartmental model and the values of rate constants of influx (K1), efflux (k2), phosphorylation, and dephosphorylation for 2DG, and K1 and k2 for 3MG were computed by nonlinear least square regression method. No significant difference in the value of K1/k2, the distribution volume of the precursor pool, was observed between 2DG and 3M...Continue Reading

References

Feb 1, 1989·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·K HerholzW D Heiss
Jun 15, 1987·Biochemical Pharmacology·A Gjedde
Aug 1, 1986·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M NedergaardN H Diemer
Jun 1, 1985·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A GjeddeG Pawlik
Nov 18, 1985·Brain Research·T T SoncrantS I Rapoport
Apr 1, 1974·Journal of Pharmacokinetics and Biopharmaceutics·H G BoxenbaumR M Elashoff
Sep 1, 1984·Journal of Neurochemistry·G PizzolatoS I Rapoport
Sep 1, 1983·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A Gjedde, N H Diemer
Jul 1, 1982·Journal of Neurochemistry·J McCullochL Sokoloff

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Citations

Jun 7, 2005·Journal of Neurochemistry·Marcelle Moulin-SallanonVicente Ibáñez
Dec 25, 2019·Chemical Communications : Chem Comm·Yinwei ChengEnmin Li
Mar 13, 2019·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Pablo BascuñanaJens P Bankstahl

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