Measurement of corneal sublayer thickness and transparency in transgenic mice with altered corneal clarity using in vivo confocal microscopy

Vision Research
J V JesterH Dwight Cavanagh

Abstract

Measurement of sublayer thickness and transparency at cellular level in the living animal are critical to understanding the role of specific transgenes and transgene products in controlling corneal development and maintenance of transparency. Using two different transgenic mouse strains having altered corneal clarity, we have evaluated the ability of in vivo confocal microscopy to measure corneal haze and localize light scattering structures. Projection of 2-D and 3-D image information identified the nature and location of light scattering within the cornea and allowed correlation of unique structural differences to transgene expression. Our findings suggest that in vivo confocal microscopy can be used to identify the effects of transgene expression on mouse corneal transparency.

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Citations

Feb 5, 2003·Experimental Eye Research·A S Verkman
Jan 8, 2005·Nephron. Experimental Nephrology·Yoshio OhnoErik I Christensen
Jan 29, 2003·Journal of Cataract and Refractive Surgery·Jay W W ChanVictor C P Woo
May 11, 2007·Experimental Eye Research·Zhao WangMortimer M Civan
Jul 17, 2009·Veterinary Ophthalmology·Thamara A CafaroHoracio M Serra
Apr 3, 2009·Clinical & Experimental Ophthalmology·Rudolf F GuthoffOliver Stachs
Jul 13, 2011·Clinical & Experimental Ophthalmology·Johanna Tukler HenrikssonJan Pg Bergmanson
Jul 7, 2015·Progress in Retinal and Eye Research·Keith M Meek, Carlo Knupp
Sep 11, 2004·Vision Research·Christine Schmucker, Frank Schaeffel
Oct 4, 2008·Ophthalmic Surgery, Lasers & Imaging : the Official Journal of the International Society for Imaging in the Eye·Masashi YuasaKazuhisa Sugiyama
Jun 13, 2019·Seminars in Ophthalmology·Thomas H DohlmanReza Dana
Oct 26, 2005·Journal of Cataract and Refractive Surgery·Madhavan S RajanJohn Marshall
Mar 4, 2014·Journal of Cataract and Refractive Surgery·Lisanne M Nibourg, Steven A Koopmans
Mar 14, 2002·The Journal of Biological Chemistry·Jay R Thiagarajah, A S Verkman
May 5, 2017·PloS One·Danilo A JesusD Robert Iskander
May 15, 2021·Scientific Reports·Adrian FilibertiDaniel J J Carr

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