Measurement of cortisol metabolites in faeces of ruminants

Veterinary Research Communications
Erich MöstlR Palme

Abstract

Twenty-one metabolites were detected in faecal samples collected after infusion of (14C)cortisol into the jugular vein of sheep. Using high-performance liquid chromatography/radiometric analysis plus mass spectrometry. One group of metabolites had molecular weights of between 302 and 308, and another group of 350, which indicates that the substances have a C19O3 or a C21O4 structure. Therefore, an enzyme immunoassay against 5beta-androstane-3alpha-ol-11,17-dione-17-CMO:BSA was established. Faecal samples were collected from 10 cows immediately after transport and then during a course in which non-invasive diagnostic procedures were being taught (course 1). For comparison, faeces were sampled from another 5 cows that were being used for teaching invasive procedures (course 2). Six cows from a university farm served as controls. In the animals used in course 1, the highest concentrations of cortisol metabolites were measured immediately after transport to the university (median value: 2.2 micromol/kg faeces). During the first 5 days at the university, the concentrations decreased to 0.52 micromol/kg (median) and remained at this level during the rest of the course. The median concentration in the samples that were taken during co...Continue Reading

Citations

Apr 2, 2003·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·S M El-BahrR Palme
Dec 30, 2004·British Poultry Science·S RettenbacherR Palme
Aug 2, 2005·Annals of the New York Academy of Sciences·Erich MöstlRupert Palme
Nov 1, 2007·Journal of Applied Animal Welfare Science : JAAWS·Nicole LawsChris Sherwin
Feb 26, 2010·Veterinary Research Communications·Birgit FlaugerErich Möstl
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