PMID: 6276474Mar 1, 1982Paper

Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker

The Journal of Investigative Dermatology
P P BradleyG Rothstein

Abstract

We examined the hypothesis that myeloperoxidase (MPO), a plentiful constituent of neutrophils, might serve as a marker for tissue neutrophil content. To completely extract MPO from either neutrophils or skin, hexadecyltrimethylammonium bromide (HTAB) was used to solubilize the enzyme. With this detergent treatment, 97.8 +/- 0.2% of total recoverable MPO was extracted from neutrophils with a single HTAB treatment; 93.1 +/- 1.0% was solubilized with a single treatment of skin. Neutrophil MPO was directly related to neutrophil number; with the dianisidine-H2O2 assay as few as 10(4) neutrophils could be detected. The background level of MPO within uninflamed tissue was 0.385 +/- 0.018 units per gram of tissue, equivalent to only 7.64 +/- 0.36 X 10(5) neutrophils. In experimental staphylococcal infection, skin specimens contained 34.8 +/- 3.8 units MPO per gram, equivalent to 8.55 +/- 0.93 X 10(7) neutrophils. These studies demonstrate that MPO can be used as a marker for skin neutrophil content: it is recoverable from skin in soluble form, and is directly related to neutrophil number. Further, normal skin possesses a low background of MPO compared to that of inflamed skin.

Citations

Sep 1, 1990·Scandinavian Journal of Clinical and Laboratory Investigation·D J ButtleM Abrahamson
Jun 1, 1989·Agents and Actions·R R HarrisN R Ackerman
Nov 1, 1993·Digestive Diseases and Sciences·B L TeppermanB D Soper
Sep 1, 1995·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·S L KaczorowskiP M Kochanek
Aug 1, 1995·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·D FretlandJ Falck
Aug 1, 1995·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·H TowbinI Wiesenberg
Aug 1, 1995·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·D E Griswold, M N Tzimas
Apr 1, 1996·Alcoholism, Clinical and Experimental Research·M PatelJ Z Fields
Jun 1, 1996·British Journal of Pharmacology·D SalveminiM G Currie
Sep 1, 1996·Scandinavian Journal of Gastroenterology·K J McHughC G Persson
Feb 1, 1997·Journal of Gastroenterology and Hepatology·Y SasayamaT Okishio
Jun 1, 1997·Scandinavian Journal of Gastroenterology·A HigaY Nawa
Sep 26, 1997·The Annals of Otology, Rhinology, and Laryngology·J R KoskoM F Pratt
Dec 31, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·S HoshidaM Hori
Mar 4, 1998·British Journal of Pharmacology·J F BrownB L Tepperman

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