Measurement of cytokine expression and Langerhans cell migration in human skin following suction blister formation

Experimental Dermatology
R J DearmanChristopher E M Griffiths

Abstract

Contact allergen-induced migration of epidermal Langerhans cells (LCs) to draining lymph nodes is dependent upon receipt by LCs of at least two cytokine signals provided by tumor necrosis factor-alpha (TNF-alpha) and interleukin (IL)-1beta. It has been reported previously that intradermal injection of healthy human volunteers with homologous TNF-alpha or IL-1beta each induces a significant reduction in LC frequency, as measured in epidermal sheets prepared from 6-mm punch biopsies. In the current experiments, we have compared the frequency of LCs in punch biopsies with those obtained concurrently in epidermal sheets from the roofs of suction blisters isolated from the sun-protected buttock skin of healthy adult volunteers. There was a significant, approximately 30%, reduction in CD1a(+) LC numbers in suction blister roofs compared with punch biopsies. Injection of homologous recombinant IL-1beta, a stimulus that provokes measurable epidermal LC mobilization in punch biopsy sites, failed to provoke further LC migration in suction blister sites. These data suggest that the mechanical trauma to the skin caused by the creation of suction blisters provokes the degree of cutaneous inflammation necessary for LC mobilization. The respo...Continue Reading

References

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Citations

Jun 22, 2006·Contact Dermatitis·Cindy M De JonghSanja Kezic
Jan 8, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Xin ZhaoStephanie J Hanna
Feb 9, 2007·Experimental Dermatology·Andrea TuettenbergHelmut Jonuleit
May 10, 2006·Clinical and Experimental Dermatology·N MacdonaldI Kimber
Oct 3, 2007·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Cindy M de JonghSanja Kezic
Aug 22, 2015·Immunology·Mohammad Alhadj AliDanijela Tatovic
Feb 22, 2011·Brain, Behavior, and Immunity·Jing HuangRichard D Granstein
Apr 5, 2013·Clinical Microbiology Reviews·Daniel GonzalezHartmut Derendorf
Nov 26, 2009·Electrophoresis·Terry M PhillipsEdward Wellner
Sep 14, 2014·EMBO Molecular Medicine·Elisabeth GlitznerMaria Sibilia

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