Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA

Journal of Chromatography
M Mesbah, W B Whitman

Abstract

The ratio of deoxyguanosine and thymidine can be determined in a complex mixture containing the major ribonucleosides and deoxynucleosides, the minor deoxynucleosides, and the nucleotide monophosphates by high-performance liquid chromatography. The isocratic procedure utilizes a C18 column and a solvent of methanol-triethylamine phosphate (pH 5.1). A single analysis requires 15 min. Within the range of 0.5-1.5 micrograms of total deoxynucleosides per sample, the determination is very precise and the relative standard deviation is about 0.1%. From the deoxyguanosine/thymidine ratio, a precise determination of the mole percentage guanine + cytosine of double-stranded DNA is calculated.

Citations

Jan 7, 2014·The Journal of Microbiology·Yochan JoungKiseong Joh
Sep 21, 2005·Applied Microbiology and Biotechnology·Norihiko TsukagoshiRyuichiro Kurane
Sep 21, 2007·Current Microbiology·Christopher RathgeberVladimir Yurkov
May 22, 2013·Current Microbiology·Yogesh M KolekarKisan M Kodam
Jul 10, 2007·Extremophiles : Life Under Extreme Conditions·Ida RomanoAgata Gambacorta
Jan 23, 2013·Antonie van Leeuwenhoek·Chunying JinWei Wang
Apr 24, 2013·Antonie van Leeuwenhoek·Ying-Yi HuoXue-Wei Xu
Mar 14, 2008·The Journal of Microbiology·Seong Woon RohJin-Woo Bae
Aug 28, 2010·The Journal of Microbiology·Yochan JoungKiseong Joh
Dec 29, 2011·The Journal of Microbiology·Yochan JoungKiseong Joh
Jul 6, 2006·Applied and Environmental Microbiology·Julius T CsotonyiVladimir Yurkov
Oct 16, 2008·Archives of Microbiology·Bo JiangAlfons J M Stams
Oct 31, 2015·International Journal of Systematic and Evolutionary Microbiology·Yan LiuXiao-Hua Zhang
Nov 1, 2015·International Journal of Systematic and Evolutionary Microbiology·Zhisong CuiLi Zheng
Oct 16, 2015·International Journal of Systematic and Evolutionary Microbiology·Yanfen ZhengXiao-Hua Zhang
Oct 18, 2015·International Journal of Systematic and Evolutionary Microbiology·Yumin ZhangFang Peng
Oct 20, 2015·International Journal of Systematic and Evolutionary Microbiology·Yumin ZhangFang Peng
Sep 17, 2015·International Journal of Systematic and Evolutionary Microbiology·Minwook KimSung-Jae Lee
Sep 12, 2015·International Journal of Systematic and Evolutionary Microbiology·Christiane Lourenço NogueiraSylvia Cardoso Leão
Sep 20, 2015·International Journal of Systematic and Evolutionary Microbiology·Pavel ŠvecIvo Sedláček
Dec 9, 2015·International Journal of Systematic and Evolutionary Microbiology·Jeesun ChunKwang-Yeop Jahng
Nov 13, 2015·International Journal of Systematic and Evolutionary Microbiology·Dildar WuXu-Fen Zhu
Nov 12, 2015·International Journal of Systematic and Evolutionary Microbiology·Zenghu ZhangXiao-Hua Zhang
Aug 22, 2015·International Journal of Systematic and Evolutionary Microbiology·Zenghu ZhangXiao-Hua Zhang
Aug 26, 2015·International Journal of Systematic and Evolutionary Microbiology·Long WangXiao-Hua Zhang
Jul 30, 2015·International Journal of Systematic and Evolutionary Microbiology·Xiao-Min FengJie Lv
Jul 23, 2015·International Journal of Systematic and Evolutionary Microbiology·Yue-Hong WuXue-Wei Xu
May 9, 2015·International Journal of Systematic and Evolutionary Microbiology·Ulrike LyhsPeter Vandamme

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