Measurement of desorption rates from octadecylsilyl bonded-phase HPLC particles and its characterization in terms of pore, surface, and film diffusion

Analytical Chemistry
Robert Bujalski, Frederick F Cantwell

Abstract

An instrument is developed to measure rates of desorption of solutes from particulate HPLC packing materials for processes that are quantitatively complete in a few tenths of a second. The instrument is a modified, pressure-driven, stopped-flow device. The major modifications include positioning a very short (0.6 mm) bed of the particles just upstream of the detector cell, eliminating the mixing chamber, and adding high-speed switching valves in order to allow sequential continuous flow of individual solutions. Instantaneous rate curves are measured for the desorption of 1,2-dimethyl-4-nitrobenzene (DMNB) from 12-microm-diameter porous particles of the bonded-phase packing Luna C-18 employing high linear velocities of the eluting solvent. The same experiment is performed for the nonsorbed compound phloroglucinol (PG) The PG rate curve is used in two ways (i.e., subtraction and deconvolution) in order to correct the observed rate curve of DMNB for experimental artifacts such as bed hold-up volume and instrument band broadening. The cumulative desorption rate curve of DMNB is obtained by integration. It is accurately described (R2 > 0.999) by a theoretical model that invokes both intraparticle diffusion (including both hindered p...Continue Reading

References

Nov 28, 2001·Analytical Chemistry·C J DunlapP W Carr
Mar 5, 2003·Journal of Chromatography. a·Victoria L McGuffin, Chomin Lee
Aug 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Stephane VielStefano Caldarelli
Aug 20, 2003·Journal of Chromatography. a·Qing DengRobert T Kennedy
Sep 11, 2003·Analytical Chemistry·Melody D LudesMary J Wirth
Dec 17, 2003·Analytical Chemistry·Chengdu LiangGeorges Guiochon
May 21, 2004·The Analyst·Luis A ColónKelly A Swinney
Feb 1, 1983·Analytical Chemistry

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Citations

Dec 14, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kiyoharu NakataniYuki Kawasaki
May 13, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kiyoharu NakataniAtsunobu Haga
Jun 13, 2015·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kiyoharu Nakatani, Emi Matsuta
Feb 14, 2017·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Tatsumi Sato, Kiyoharu Nakatani
Mar 12, 2009·Journal of Separation Science·Kanji Miyabe
May 13, 2017·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Tatsumi SatoKiyoharu Nakatani
Jul 10, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Toshiki OtakaKiyoharu Nakatani
Jan 29, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Meiqin ZhangPatrick R Unwin
Nov 6, 2007·Analytical Chemistry·M BarrandeR Denoyel

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