Measurement of dimensions of pentagonal doughnut-shaped C-reactive protein using an atomic force microscope and a dual polarisation interferometric biosensor

Biosensors & Bioelectronics
Shiming LinSu-Ming Hsu

Abstract

In order to develop the C-reactive protein (CRP) sensor chips for clinical detection of atherosclerosis and coronary heart disease, we used an atomic force microscope (AFM) and a dual polarization interferometric (DPI) biosensor to probe the surface ultrastructure and to measure the dimensions of CRP. A single pentagonal structure was directly visualized by AFM, and quantitative measurements of the dimensions of the protein were provided. The average height calculated for each pentagonal CRP particle was approximately 3.03+/-0.37 nm, which basically corresponds to that (36 A in protomer diameter) previously obtained from the structure of CRP determined by X-ray crystallography. Moreover, a experiment using dual polarization interferometric (DPI) as a biosensor was then performed, and the average monolayer thickness value (3.18+/-0.43 nm) that was calculated basically corresponds to that obtained from the experimental value (3.03+/-0.37 nm) of the height measured by an AFM method for CRP. Further investigations will be performed to study the surface ultrastructure of a single pentagonal CRP molecule, and for this purpose a CRP sample (at low concentration) was scanned in vacuum by AFM. The higher-resolution images clearly reveal...Continue Reading

References

Apr 1, 1996·Nature Structural Biology·A K ShriveT J Greenhough
Mar 3, 1986·Physical Review Letters·G BinnigC Gerber
Sep 5, 2001·Molecular Immunology·J E Volanakis
Oct 19, 2002·Critical Reviews in Clinical Laboratory Sciences·Laura R BenzaquenNader Rifai
Jan 14, 2003·Biophysical Journal·J C SitkoH G Hansma
Nov 15, 2003·Biosensors & Bioelectronics·Graham H CrossNeville J Freeman
Nov 26, 2003·Circulation·Gavin J BlakePaul M Ridker
Jan 6, 2004·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·R P Tracy
Aug 7, 2004·The EMBO Journal·Daniel M CzajkowskyRodney K Tweten
Aug 19, 2004·Journal of Cellular Physiology·Victor ShahinHans Oberleithner
Mar 1, 2005·Journal of Molecular Biology·Yurii G KuznetsovA McPherson
Apr 2, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Jian R LuNeville J Freeman

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Citations

Oct 9, 2012·Biosensors & Bioelectronics·Pingping XuHaojun Liang
Feb 23, 2008·Annals of Medicine·Sanjay K SinghAlok Agrawal
Oct 8, 2011·Nanotechnology·Mateusz SikoraMarek Cieplak
Apr 28, 2009·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Osbert Tan, Graham H Cross
Jul 18, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Qiqiang Wang, Kam C Tam
Apr 25, 2012·The Journal of Physical Chemistry. B·David Giménez-RomeroÁngel Maquieira
Dec 3, 2014·Chemical Reviews·Jorge EscorihuelaDavid Gimenez-Romero

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