Measurement of fatty acid oxidation: validation of isotopic equilibrium extrapolation

Metabolism: Clinical and Experimental
A P RobinJ M Kinney

Abstract

Measurement of whole body substrate oxidation requires prolonged isotope infusion to attain plateau specific activity (SA) of expired CO2. We have investigated in 13 hospitalized patients a technique whereby plateau 14CO2 SA is extrapolated using computer curve fitting based upon the early exponential rise. A primed-constant infusion of albumin-bound 1-14C-palmitate was continued for 260 minutes with isotope priming of the secondary bicarbonate pool at 70 minutes. Plasma free fatty acid (FFA) SA reached steady state by 40 minutes and was 91% +/- 4% (SE) of values obtained at 190 to 260 minutes. At 70 minutes 14CO2 SA reached only 44% +/- 1% of the 190 to 260 minute values, which were consistently at plateau. The predicted steady state 14CO2 SA from the 40 to 70 minute curves and the FFA oxidation rates calculated from those values were 94% +/- 2% and 102% +/- 4%, respectively, of values measured at steady state (190 to 260 minutes). The relationship between predicted and measured values approximated the line of identity for 14CO2 SA (y = 0.90x + 0.14, r = .98, P less than .001) and FFA oxidation (y = 1.02x, r = .98, P less than .001). The results suggest that FFA oxidation can be accurately calculated using a short infusion of ...Continue Reading

References

Jul 1, 1978·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·J R AllsopJ F Burke
Apr 1, 1977·Biomedical Mass Spectrometry·D Halliday, A G Miller
Feb 1, 1987·The Journal of Clinical Investigation·J A WisneskiM Mayr
Nov 2, 1974·Lancet·S J O'KeefeW P James
Feb 1, 1969·Clinica Chimica Acta; International Journal of Clinical Chemistry·P F Dixon
Oct 1, 1972·Journal of Applied Physiology·J L SpencerA B Lee
Jul 1, 1969·Metabolism: Clinical and Experimental·P Paul, W M Bortz
Jul 1, 1971·Journal of Applied Physiology·C L LongJ W Geiger
Jul 1, 1971·Journal of Applied Physiology·C L LongJ W Geiger
Jan 1, 1968·Metabolism: Clinical and Experimental·B IssekutzW M Bortz
Feb 1, 1954·The American Journal of Physiology·G L SEARLEI L CHAIKOFF
Jul 1, 1955·The Biochemical Journal·R STEELE
Oct 1, 1956·The American Journal of Physiology·R STEELEN ALTSZULER
Nov 1, 1960·The American Journal of Medicine·D B ZILVERSMIT
Jan 1, 1964·The American Journal of Physiology·M GOLD, J J SPITZER

❮ Previous
Next ❯

Citations

Jan 1, 1991·The Journal of Clinical Investigation·L C GroopR A DeFronzo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.