Measurement of functional microcirculatory geometry and velocity distributions using automated image analysis.

Medical & Biological Engineering & Computing
Johannes G DobbeCan Ince

Abstract

This study describes a new method for analyzing microcirculatory videos. It introduces algorithms for quantitative assessment of vessel length, diameter, the functional microcirculatory density distribution and red blood-cell (RBC) velocity in individual vessels as well as its distribution. The technique was validated and compared to commercial software. The method was applied to the sublingual microcirculation in a healthy volunteer and in a patient during cardiac surgery. Analysis time was reduced from hours to minutes compared to previous methods requiring manual vessel identification. Vessel diameter was detected with high accuracy (>80%, d > 3 pixels). Capillary length was estimated within 5 pixels accuracy. Velocity estimation was very accurate (>95%) in the range [2.5, 1,000] pixels/s. RBC velocity was reduced by 70% during the first 10 s of cardiac luxation. The present method has been shown to be fast and accurate and provides increased insight into the functional properties of the microcirculation.

References

Jun 1, 1997·Biomedizinische Technik. Biomedical Engineering·T KlysczH Zeintl
Mar 18, 2000·Journal of Vascular Research·A S De VrieseP M Vanhoutte
Dec 1, 2001·Lancet·K R MathuraC Ince
Feb 28, 2002·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·P Biberthaler, S Langer
Jul 2, 2002·American Journal of Respiratory and Critical Care Medicine·Daniel De BackerJean-Louis Vincent
Aug 21, 2002·Journal of Vascular Research·J LindertA R Pries
Nov 9, 2002·Lancet·Peter E SpronkDurk F Zandstra
Apr 16, 2004·IEEE Transactions on Medical Imaging·Joes StaalBram van Ginneken
Apr 17, 2004·Stroke; a Journal of Cerebral Circulation·Frederik A PenningsCan Ince
Jul 29, 2004·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Shruti A JapeeRoland N Pittman
Sep 7, 2004·Pediatric Research·Orsolya Genzel-BoroviczényVerena Glas
Sep 9, 2005·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Shruti A JapeeChristopher G Ellis
Sep 20, 2005·Critical Care : the Official Journal of the Critical Care Forum·Can Ince
Nov 11, 2005·Critical Care : the Official Journal of the Critical Care Forum·E Christiaan BoermaCan Ince
May 23, 2006·Critical Care Medicine·Daniel De BackerJean-Louis Vincent
Nov 11, 2006·Annals of Emergency Medicine·Stephen TrzeciakUNKNOWN Microcirculatory Alterations in Resuscitation and Shock Investigators
Sep 12, 2007·Critical Care : the Official Journal of the Critical Care Forum·Daniel De BackerCan Ince
Jun 1, 1986·IEEE Transactions on Pattern Analysis and Machine Intelligence·J Canny

❮ Previous
Next ❯

Citations

May 8, 2008·Intensive Care Medicine·Can Ince
Jan 8, 2009·Intensive Care Medicine·Arnaldo DubinCan Ince
Dec 25, 2012·Intensive Care Medicine·Andrius PranskunasE Christiaan Boerma
Apr 1, 2009·European Journal of Applied Physiology·Daniel S MartinUNKNOWN Caudwell Xtreme Everest Research Group
Feb 9, 2012·Clinical Rheumatology·Ming-Yii HuangFei-Kai Syu
Sep 1, 2011·Medical & Biological Engineering & Computing·Rick BezemerCan Ince
Apr 26, 2012·Medical & Biological Engineering & Computing·Michela GoffredoClaudio Palma
Nov 18, 2010·Computer Methods in Biomechanics and Biomedical Engineering·Wen-Chen LinKang-Ping Lin
Dec 19, 2012·BMJ : British Medical Journal·Can InceArnoldus S Blix
Jul 16, 2010·BMC Medical Imaging·Gianmarco M BalestraCan Ince
Jun 21, 2012·Critical Care : the Official Journal of the Critical Care Forum·Rick BezemerCan Ince
Apr 5, 2011·Critical Care : the Official Journal of the Critical Care Forum·Anke P C TopCan Ince
Jul 4, 2008·Pediatric Research·Julia KrothOrsolya Genzel-boroviczény
Feb 18, 2014·Critical Care : the Official Journal of the Critical Care Forum·Abele DonatiCan Ince
Jun 27, 2012·Biomarkers in Medicine·Erik A B BuijsSaskia N de Wildt
Jul 30, 2009·Intensive Care Medicine·Corstiaan A den UilMaarten L Simoons
May 21, 2014·Microvascular Research·Jan NilssonChristian Sturesson
Jun 6, 2014·Clinical Research in Cardiology : Official Journal of the German Cardiac Society·Anna SelleAlexander Lauten
Sep 17, 2013·Journal of Cardiothoracic and Vascular Anesthesia·Nick J KoningChrista Boer
Mar 10, 2010·Journal of Biomedical Optics·Beatriz Y Salazar VázquezMarcos Intaglietta
Feb 11, 2016·Critical Care : the Official Journal of the Critical Care Forum·Michael J Massey, Nathan I Shapiro
Jan 23, 2016·BMC Medical Imaging·Edward Gilbert-KawaiUNKNOWN Caudwell Xtreme Everest and Xtreme Everest 2 Research Groups
May 18, 2012·Journal of Cardiothoracic and Vascular Anesthesia·Nihal Gökbulut ÖzarslanÜlkü Aypar
Mar 6, 2012·Journal of Cardiothoracic and Vascular Anesthesia·Alessandro FortiCarlo Sorbara
Aug 30, 2011·The American Journal of Emergency Medicine·Christopher J HoganLeroy R Thacker
Jan 25, 2011·Microvascular Research·Chih-Chieh WuTzung-Chi Huang
Jan 26, 2010·Journal of Cardiothoracic and Vascular Anesthesia·Bektaş AtaseverCan Ince
Aug 13, 2015·Journal of Critical Care·Namkje A R VellingaE Christiaan Boerma
Sep 1, 2009·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Corstiaan A den UilMaarten L Simoons
Sep 9, 2008·Progress in Cardiovascular Diseases·Corstian A den UilM L Simoons
Feb 13, 2009·European Journal of Heart Failure·Corstiaan A den UilMaarten L Simoons
Apr 27, 2010·Experimental Physiology·Daniel S MartinUNKNOWN Caudwell Xtreme Everest Research Group
Aug 16, 2014·Journal of Veterinary Emergency and Critical Care·Kris GommerenDeborah C Silverstein

❮ Previous
Next ❯

Methods Mentioned

BETA
imaging techniques
coronary artery

Software Mentioned

CapImage
CapiScope

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.