PMID: 14399016Jul 1, 1960Paper

Measurement of globular protein molecules by electron microscopy

The Journal of Biophysical and Biochemical Cytology
C E HALL

Abstract

A series of molecular species with approximately spherical shape and with molecular weights between 35,000 and 250,000 were shadowed with platinum while resting on a cleaved mica surface. They were backed, stripped from the surface, and examined by electron microscopy. Materials examined were: pepsin, liver alcohol dehydrogenase, yeast alcohol dehydrogenase, glutamic dehydrogenase, polyhedral virus protein (insect), fibrinogen substructure, alkaline phosphatase, and microsomal particles from Escherichia coli. Measurements were made of widths perpendicular to the shadowing direction and heights were deduced from shadow lengths. For those molecular species with well established molecular weights the average heights correlate very well with the diameter of the theoretical sphere but the average widths are too great by 50 to 80 A due to the lateral growth of the deposited metal. Although the distortion in shape of shadowed particles is relatively large, with standardized conditions for shadowing, it is possible to make allowance for the distortion and to obtain reasonably reliable estimates of the dimensions of spherical organic particles down to a molecular weight of about 35,000.

References

Sep 25, 1956·The Journal of Biophysical and Biochemical Cytology·C E HALL
Jan 25, 1959·The Journal of Biophysical and Biochemical Cytology·C E HALL, H S SLAYTER

❮ Previous
Next ❯

Citations

Mar 1, 1970·Virology·M E Bayer, C C Remsen
Dec 8, 1998·Micron : the International Research and Review Journal for Microscopy·G C Ruben
May 1, 1961·The Journal of Biophysical and Biochemical Cytology·S N CHATTERJEEJ B CHATTERJEA
Jan 1, 1962·The Journal of Cell Biology·I OHADS HESTRIN
Jul 1, 1964·The Journal of Cell Biology·I OHAD, D DANON
Feb 1, 1966·The Journal of Cell Biology·J A Carney, A L Brown
Dec 1, 1970·The Journal of Cell Biology·N S McNutt, R S Weinstein
Jan 1, 1974·The Journal of Cell Biology·J T StasnyR W Hardy
Dec 1, 1964·J R Microsc Soc·R A MALTE BELL
May 12, 2010·British Journal of Haematology·Gang ZhiGary M Kupfer
Aug 1, 1973·Virology·M E Bayer, A F Bocharov
Feb 1, 1966·The Journal of Social Psychology·G D Rastogi
May 25, 1962·Biochemistry·D J PLOCKEB L VALLEE
Oct 1, 1968·Experimental Cell Research·A Engström, R Kilkson
Apr 1, 1963·Journal of Ultrastructure Research·G H BERGOLD
Dec 1, 1965·Experimental Eye Research·H BloemendalJ H Wisse
Sep 1, 1976·Ultramicroscopy·H S Slayter
Jul 13, 1963·Nature·K DEUTSCH
Jul 14, 1962·Nature·K DEUTSCHA KALAIDJIEV
Dec 1, 1984·Journal of Bioenergetics and Biomembranes·J N TelfordE Racker
Jan 1, 1973·Progress in Biophysics and Molecular Biology·N S McNutt, R S Weinstein
Aug 28, 1968·Journal of Molecular Biology·L W Labaw, J E Rall
Dec 1, 1979·Journal of Ultrastructure Research·F S Sjöstrand
Jan 14, 1968·Journal of Molecular Biology·J E Mellema, E F van Bruggen
Jun 1, 1963·Journal of Molecular Biology·R W HORNE, G D GREVILLE

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.