Measurement of gluconeogenesis using glucose fragments and mass spectrometry after ingestion of deuterium oxide

Journal of Applied Physiology
Shaji K ChackoMorey W Haymond

Abstract

We report a new method to measure the fraction of glucose derived from gluconeogenesis using gas chromatography-mass spectrometry and positive chemical ionization. After ingestion of deuterium oxide by subjects, glucose derived from gluconeogenesis is labeled with deuterium. Our calculations of gluconeogenesis are based on measurements of the average enrichment of deuterium on carbon 1, 3, 4, 5, and 6 of glucose and the deuterium enrichment in body water. In a sample from an adult volunteer after ingestion of deuterium oxide, fractional gluconeogenesis using the "average deuterium enrichment method" was 48.3 +/- 0.5% (mean +/- SD) and that with the C-5 hexamethylenetetramine (HMT) method by Landau et al. (Landau BR, Wahren J, Chandramouli V, Schumann WC, Ekberg K, Kalhan SC; J Clin Invest 98: 378-385, 1996) was 46.9 +/- 5.4%. The coefficient of variation of 10 replicate analyses using the new method was 1.0% compared with 11.5% for the C-5 HMT method. In samples derived from an infant receiving total parenteral nutrition, fractional gluconeogenesis was 13.3 +/- 0.3% using the new method and 13.7 +/- 0.8% using the C-5 HMT method. Fractional gluconeogenesis measured in six adult volunteers after 66 h of continuous fasting was 83...Continue Reading

References

Nov 1, 1992·The American Journal of Physiology·M K Hellerstein, R A Neese
May 1, 1987·The American Journal of Physiology·G M ArgoudR P Eaton
Sep 1, 1974·European Journal of Biochemistry·R RognstadJ Katz
Jan 1, 1995·The Journal of Clinical Investigation·B R LandauS C Kalhan
Jul 15, 1996·The Journal of Clinical Investigation·B R LandauS C Kalhan
Sep 1, 1997·The Journal of Clinical Investigation·M K HellersteinA Letscher
Sep 2, 1998·The American Journal of Physiology·J Katz, J A Tayek
Jan 25, 2000·American Journal of Physiology. Endocrinology and Metabolism·M W Haymond, A L Sunehag
Sep 5, 2002·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·John G JonesCarlos F G C Geraldes
Nov 5, 2002·The Journal of Clinical Endocrinology and Metabolism·Agneta L SunehagMorey W Haymond
Jun 2, 2005·The Journal of Clinical Endocrinology and Metabolism·Agneta L SunehagMorey W Haymond

❮ Previous
Next ❯

Citations

Dec 31, 2008·The Journal of Biological Chemistry·Ilya R BedermanStephen F Previs
Aug 6, 2009·American Journal of Physiology. Endocrinology and Metabolism·Mahmoud A MohammadMorey W Haymond
Oct 4, 2012·Critical Care : the Official Journal of the Critical Care Forum·Carlijn T I de BetueKoen F M Joosten
Oct 8, 2009·The Journal of Clinical Endocrinology and Metabolism·Gert-Jan van der HeijdenAgneta L Sunehag
Mar 31, 2010·Medicine and Science in Sports and Exercise·Gert-Jan Van Der HeijdenAgneta L Sunehag
Nov 26, 2015·Diabetes·Stephanie T ChungMorey W Haymond
Jul 23, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Ivan ViegasJohn G Jones
Jul 2, 2011·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Sascha C A T VerbruggenKoen F M Joosten
Dec 3, 2014·Diabetologia·Stephanie T ChungMorey W Haymond
Feb 27, 2016·The Journal of Physiology·Christopher C WebsterJames A H Smith
Jun 20, 2018·American Journal of Physiology. Endocrinology and Metabolism·Claire GaudichonJean-Philippe Godin
Apr 15, 2010·Rapid Communications in Mass Spectrometry : RCM·Peter JunghansCornelia C Metges
Feb 18, 2012·American Journal of Physiology. Endocrinology and Metabolism·Shaji K ChackoAgneta L Sunehag
Jun 19, 2012·Experimental Physiology·Ilya R BedermanMarco E Cabrera
Jun 16, 2017·Science Translational Medicine·Annika S AxelssonAnders H Rosengren
Mar 20, 2020·The Journal of Clinical Investigation·Hilal SekizkardesRebecca J Brown
Aug 29, 2018·Obesity Science & Practice·M A B VeldhorstE L T van den Akker
Aug 27, 2019·Frontiers in Endocrinology·Chellakkan S BlessonChandra Yallampalli
Sep 21, 2018·JCI Insight·Stephanie T ChungAnne E Sumner
Mar 27, 2019·American Journal of Physiology. Endocrinology and Metabolism·Natalie A DaurioStephen F Previs
Oct 26, 2021·American Journal of Physiology. Endocrinology and Metabolism·Emmanuel QuayeRebecca J Brown

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.