Measurement of Growth at Very Low Rates ((mu) >= 0), an Approach To Study the Energy Requirement for the Survival of Alcaligenes eutrophus JMP 134.

Applied and Environmental Microbiology
R H Muller, W Babel

Abstract

Alcaligenes eutrophus JMP 134 was grown in a recycling-mode fermenter with 100% biomass retention on 2,4-dichlorophenoxyacetic acid (2,4-D), phenol, and fructose. The growth pattern obtained given a constant supply of substrates exhibited three phases of linear growth on all three substrates. The transition from phase 1 to phase 2, considered to correspond to the onset of stringent (growth) control as indicated by a significant increase in guanosine 5(prm1)-bisphosphate 3(prm1)-bisphosphate (ppGpp), took place at 0.016 h(sup-1) with 2,4-D and at about 0.02 h(sup-1) with phenol and fructose. In the final phase, phase 4, which was achieved after the growth rate on the respective substrates fell below 0.003 to 0.001 h(sup-1), a constant level of biomass was obtained irrespective of further feeding of substrate at the same rate. The yield coefficients decreased by 70 to 80% from phase 1 to phase 3 and were 0 in phase 4. The stationary substrate concentrations s(infmin) in phase 4, calculated from the kinetic constants of the strain, were 1.23, 0.34, and 0.23 (mu)M for 2,4-D, phenol, and fructose, respectively. These figures characterize the minimum stationary substrate concentrations required in a dynamic system to keep A. eutrophu...Continue Reading

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Citations

Oct 13, 2006·Bioprocess and Biosystems Engineering·Martin KollerGerhart Braunegg
Sep 25, 2010·Molecular Systems Biology·Philippe GoffinJeroen Hugenholtz
Jun 30, 2009·Applied and Environmental Microbiology·Mark M DavidsonTullis C Onstott
Oct 3, 2006·Biotechnology and Bioengineering·Anja Drews, Matthias Kraume
Jan 1, 2010·Annual Review of Food Science and Technology·E J Smid, J Hugenholtz
Feb 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·B P WeissK H Nealson
Apr 1, 1997·Ecotoxicology and Environmental Safety·N LoffhagenW Babel
Sep 1, 1996·Applied and Environmental Microbiology·A KonopkaR F Turco

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