PMID: 8601325Apr 1, 1996Paper

Measurement of human erythrocyte CAI and CAII in adult, newborn, and fetal blood

Clinical Biochemistry
S Aliakbar, P R Brown

Abstract

The aim of the present study was to determine the erythrocyte CAI and CAII concentrations in fetal blood over a wide gestational range, and compare levels to those found in neonates and adults. Human erythrocyte CAI and CAII concentrations were measured in fetal (n=38), neonatal (n=10) and adult (n=30) blood, using enzyme-linked immunosorbent assays (ELISA). For the measurement of CAII, a new ELISA method was developed. The ELISA method was found to be simple, sensitive, economical, and precise. The normal mean levels of erythrocyte CAI and CAII in adults with standard deviation were 13.68 +/- 2.79, and 1.59 +/- 0.21 mg/g Hb, respectively. The corresponding values in cord blood obtained at delivery at 38-40 weeks gestation were 1.20 +/- 0.68 and 0.46 +/- 0.13 mg/g Hb. The mean CAI/CAII ratio in adults was 8.8 and, in newborns, it was 2.5. The normal mean fetal erythrocyte CAI concentration increased significantly with gestation from 39 microgram/g Hb at 20 weeks to 380 microgram/g Hb at 38 weeks of gestation. Similarly, the CAII concentration increased from 53 microgram/g Hb at 20 weeks to 120 microgram/g Hb at 38 weeks of gestation. The CAI to CAII ratio also increased with gestation from 0.9 at 20 weeks to 2.5 at 38 weeks.

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Citations

Feb 1, 1996·Clinical Biochemistry·S A Akbar, P R Brown
Dec 17, 2003·Clinical Biochemistry·Denise Mafra, Silvia M F Cozzolino
Sep 27, 2003·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·B L TuftsS G Lund
Mar 18, 2009·Journal of Perinatal Medicine·Christoph ScholzFranz Kainer
Apr 20, 2000·American Journal of Veterinary Research·T NishitaM Asari
Nov 12, 2005·European Journal of Clinical Investigation·R Baumann, S Dragon
Jun 29, 2012·Transfusion·Eugenia Prus, Eitan Fibach
Nov 27, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Wu-Hsien KuoShu-Chen Chu
Feb 9, 2005·The Journal of Veterinary Medical Science·Toshiho NishitaMasao Asari
Aug 6, 2016·Fetal and Pediatric Pathology·Mariela Granero FariasSergio H Almeida Martins Costa
Sep 22, 2018·Prenatal Diagnosis·Sasha SkinnerRyan Hodges
Sep 6, 2017·Archives of Physiology and Biochemistry·Gülten ErkenNahit Gencer
Oct 3, 2012·Cytometry. Part B, Clinical Cytometry·Cedric PastoretMikael Roussel
Apr 2, 2019·BioMed Research International·Marcella R CardosoRicardo Barini
Feb 26, 2021·Current Opinion in Hematology·Eugene Khandros, Gerd A Blobel

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