PMID: 489991Jan 1, 1979Paper

Measurement of immunoglobulin binding to synovial fibroblast monolayers: comparison of staphylococcal protein A binding to cytotoxic assay methods

Journal of Immunological Methods
W B Gruhn, F C McDuffie

Abstract

We have studied the binding of rabbit antibody to cultured synovial fibroblasts by 3 methods: complement mediated cell lysis, 125I-labeled staphylococcal protein A ([125I]PrA) and antibody dependent cell mediated cytotoxicity (ADCC). The relative sensitivities of these 3 methods is 1 : 33 : 3300 respectively. The [125I]PrA binding method is shown to quantitatively detect cell bound IgG with a molar stoichiometry of approximately 1 : 1, protein A (PrA) to IgG if PrA is present in excess.

References

Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·P Bornstein, J F Ash
Sep 1, 1976·The Journal of Clinical Investigation·M M GriffithsM R Klauber
Sep 1, 1976·The Journal of Clinical Investigation·D A PersonM D Lidsky
Nov 11, 1976·Arthritis and Rheumatism·R C WilliamsJ D Montaño
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Sep 25, 1972·European Journal of Biochemistry·J SjöquistH Hjelm
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Sep 1, 1965·Immunochemistry·G ManciniJ F Heremans
Jan 1, 1968·Scandinavian Journal of Clinical and Laboratory Investigation·C E Mogensen

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Citations

Mar 30, 1984·Journal of Immunological Methods·B HeymanU Heyman
Jun 1, 1982·American Journal of Hematology·P YamP Spath

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