PMID: 6406601May 27, 1983Paper

Measurement of K cell activity: use of human OR1R1 red cells and anti-D to study the role of different leucocytes as effector cells

Journal of Immunological Methods
A K SinghG Wetherley-Mein

Abstract

A method for quantitative assay of K cell activity (KCA) with an allogenic combination of human OR1R1 red cells and anti-D (IgG, incomplete antibody) as target cells is described. Lymphocytes alone have been shown to cause lysis of these target cells. Fc receptor-bearing lymphocytes appear to be the chief mediators of KCA. This sub-population is a heterogeneous mixture of sub-sets of other sub-populations defined on the basis of different surface markers and likely to show variable expression of Fc receptors by the EA gamma rosette method. Monocytes and neutrophils (and possibly eosinophils), most of which bear Fc receptors, are ineffective as effector cells. Monocytes may in some instances have an inhibitory effect on the expression of KCA of lymphocytes. The method described has the advantage of simplicity (easy availability of OR1R1 cells and anti-D) and a high degree of sensitivity (marked lysis at low L/T ratio) and appears suitable for routine use in clinical practice.

References

Dec 1, 1978·British Journal of Haematology·A K SinghG Wetherley-Mein
Mar 1, 1977·European Journal of Immunology·M H van OersP T Schellekens
Jan 1, 1975·European Journal of Immunology·M FerrariniM L Durante
Oct 16, 1971·Lancet·M PapamichailE J Holborow
Jul 1, 1972·The Journal of Experimental Medicine·H B Dickler, H G Kunkel
Jul 15, 1972·Lancet
Jan 1, 1968·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·H Wetterqvist
Sep 1, 1970·Cellular Immunology·P Perlmann, H Perlmann

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Citations

May 4, 2004·Blood Cells, Molecules & Diseases·Gaëtan DecherfSerge L Thomas

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