Measurement of lacunar bone strains and crack formation during tensile loading by digital volume correlation of second harmonic generation images

Journal of the Mechanical Behavior of Biomedical Materials
Scott WentzellShiva Kotha

Abstract

The maintenance of healthy bone tissue depends upon the ability of osteocytes to respond to mechanical cues on the cellular level. The combination of digital volume correlation and second harmonic generation microscopy offers the opportunity to investigate the mechanical microenvironment of intact bone on the scale of individual osteocytes. Adult human femurs were imaged under tensile loads of 5 and 15MPa and volumes of approximately 492×429×31μm(3) were analyzed, along with an image of a bone microcrack under the same loading conditions. Principal strains were significantly higher in three-dimensional digital volume correlation when compared to two-dimensional digital image correlation. The average maximum principal strain magnitude was 5.06-fold greater than the applied global strain, with peak strains of up to 23.14-fold over global strains measured at the borders of osteocyte lacunae. Finally, a microcrack that initiated at an osteocyte lacunae had its greatest tensile strain magnitudes at the crack expansion front in the direction of a second lacunae, but strain at the crack border was reduced to background strain magnitudes upon breaching the second lacunae. This serveed to demonstrate the role of lacunae in initiating, m...Continue Reading

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Jul 13, 2013·Journal of Biomechanics·Scott WentzellShiva Kotha

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Citations

Jan 4, 2017·The Review of Scientific Instruments·Maodong RenBin Wei

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