Measurement of leukemia inhibitory factor in biological fluids by radioimmunoassay

The Journal of Clinical Endocrinology and Metabolism
S G RenS Melmed

Abstract

Leukemia inhibitory factor (LIF) exhibits multiple biological activities in various tissues, and we have shown that LIF activates POMC gene transcription in response to immune signals. As higher serum levels of LIF have been reported in septicemia, we measured LIF values in biological fluids by RIA. Immunoreactive LIF was detected in 303 of 428 human serum samples. Circulating LIF detection rates were 69% in acute inflammatory diseases, 83% in chronic inflammatory diseases, 61% in noninflammatory diseases, and 90% in cancer patients. Serum concentrations of human LIF was higher in patients with inflammatory disease than in noninflammatory disease (0.80 +/- 0.10 vs. 0.53 +/- 0.02 ng/mL; P < 0.05) or in cancer patients (0.44 +/- 0.06; P < 0.05). Higher serum human LIF levels were found in septicemia (0.78 +/- 0.14 ng/mL), pneumonia (0.80 +/- 0.10 ng/mL), acute bronchitis (0.88 +/- 0.09 ng/mL), other infections (1.01 +/- 0.17 ng/mL), and systemic lupus erythematosus (SLE; 0.79 +/- 0.06 ng/mL). In 7 septicemia patients, Gram-negative infection was associated with higher LIF levels (1.06 +/- 0.16 ng/mL) than was Gram-positive infection (0.58 +/- 0.14 ng/mL). In patients with acute inflammatory disease, serum LIF levels decreased wit...Continue Reading

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Citations

Aug 25, 2010·Current Opinion in Supportive and Palliative Care·Stephanie M Krasnow, Daniel L Marks
Jan 15, 2008·American Journal of Respiratory Cell and Molecular Biology·Lee J QuintonJoseph P Mizgerd
May 13, 2006·Endocrinology·Song-Guang Ren, Shlomo Melmed
Nov 7, 2003·The Journal of Clinical Endocrinology and Metabolism·Song-Guang RenShlomo Melmed
Sep 22, 2010·Arquivos brasileiros de endocrinologia e metabologia·Andréa Name Colado SimãoIsaias Dichi
Aug 30, 2012·Hemodialysis International·Marlene S B GonçalvesDécio S Barbosa
Jan 1, 2014·Molecular & Cellular Oncology·Shu-Chen Liu, Yu-Sun Chang
Sep 22, 2015·Frontiers in Cellular Neuroscience·Iva BozicIrena Lavrnja
Aug 24, 2019·Jornal brasileiro de nefrologia : ʹorgão oficial de Sociedades Brasileira e Latino-Americana de Nefrologia·Juliana Carvalho Romagnolli PlastinaVinicius Daher Alvares Delfino
Apr 18, 2018·Clinical Hemorheology and Microcirculation·Muzaffer AkkocaEmine Demirel-Yilmaz
Jun 4, 2019·Journal of Medical Biochemistry·Aleksandra KlisicAna Ninic

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