PMID: 5257964May 1, 1969Paper

Measurement of ligand-induced conformational changes in hemoglobin by circular dichroism

Proceedings of the National Academy of Sciences of the United States of America
S R Simon, C R Cantor

Abstract

The UV circular-dichroism spectra of human and horse hemoglobins have been determined at various degrees of partial saturation with oxygen. Spectra of the two native hemoglobins were compared with spectra of the corresponding proteins modified with a reagent known to eliminate the conformational rearrangement normally associated with cooperativity. Such comparison indicates that one region, around 260 mmu, is sensitive chiefly to the state of the hemes; changes in another region, around 285 mmu, may be correlated with the conformational transformation linked to cooperative interactions. All circular-dichroism changes are strictly linear with fractional saturation with oxygen. Possible implications of these results to recently proposed mechanisms for cooperativity in proteins are discussed.

References

Aug 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·S R Simon, W H Konigsberg
Oct 14, 1968·Journal of Molecular Biology·K Javaherian, S Beychok
Jun 1, 1964·Comparative Biochemistry and Physiology·G L SPOEKH P WOLVEKAMP
Apr 1, 1965·Analytical Biochemistry·R BENESCHR E BENESCH
May 1, 1965·Journal of Molecular Biology·J MONODJ P CHANGEUX

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Citations

Jul 1, 1975·Quarterly Reviews of Biophysics·R G ShulmanS Ogawa
Oct 26, 1977·Biochimica Et Biophysica Acta·C E Chapman, E R Simons
Jan 1, 1975·Progress in Biophysics and Molecular Biology·J M Baldwin
Sep 5, 1980·Journal of Molecular Biology·J C ChienK H Mayo
Dec 3, 1971·Biochemical and Biophysical Research Communications·M WaksS Beychok
Aug 7, 1972·Biochemical and Biophysical Research Communications·T Asakura
Dec 5, 1979·Journal of Molecular Biology·F W Snyder, J C Chien
Sep 2, 1975·Biochemical and Biophysical Research Communications·F A Ferrone, W C Topp
Jan 1, 2005·Biophysical Journal·Qiuying ChenRhoda Elison Hirsch
Jun 6, 1977·Biochemical and Biophysical Research Communications·C F Plese, E L Amma
Dec 1, 1974·FEBS Letters·J M Salhany
Aug 1, 1970·European Journal of Biochemistry·K Gersonde, A Wollmer
Apr 23, 2011·International Journal of Biological Macromolecules·Estelle Yaba AdjeNaïma Nedjar-Arroume
Jul 19, 2013·The Journal of Physical Chemistry. B·Shigenori NagatomoTeizo Kitagawa

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