Measurement of local temperature increments induced by cultured HepG2 cells with micro-thermocouples in a thermally stabilized system

Scientific Reports
Fan YangShengyong Xu

Abstract

To monitor the temperature distribution of a cell and its changes under varied conditions is currently a technical challenge. A variety of non-contact methods used for measuring cellular temperature have been developed, where changes of local temperature at cell-level and sub-cell-level are indirectly calculated through the changes in intensity, band-shape, bandwidth, lifetime or polarization anisotropy of the fluorescence spectra recorded from the nano-sized fluorescent materials pre-injected into the target cell. Unfortunately, the optical properties of the fluorescent nano-materials may be affected by complicated intracellular environment, leading to unexpected measurement errors and controversial arguments. Here, we attempted to offer an alternative approach for measuring the absolute increments of local temperature in micro-Testing Zones induced by live cells. In this method, built-in high-performance micro-thermocouple arrays and double-stabilized system with a stability of 10 mK were applied. Increments of local temperature close to adherent human hepatoblastoma (HepG2) cells were continuously recorded for days without stimulus, showing frequent fluctuations within 60 mK and a maximum increment by 285 mK. This method may...Continue Reading

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Citations

Sep 12, 2017·Chemical Communications : Chem Comm·Seiichi UchiyamaNoriko Inada
May 19, 2020·The Journal of General Physiology·Kotaro OyamaNorio Fukuda
Mar 15, 2019·Biomicrofluidics·Fan YangShengyong Xu
Mar 17, 2020·Biophysics Reviews·Madoka Suzuki, Taras Plakhotnik
Aug 3, 2019·Communications Biology·Manjunath C RajagopalSanjiv Sinha
Dec 9, 2020·Entropy·Namkyu Lee, Simone Wiegand
Nov 17, 2020·ACS Sensors·Fangxu WangNing Gu
Jan 12, 2020·Communications Biology·Manjunath C RajagopalSanjiv Sinha
May 27, 2021·ACS Sensors·Tongtong ZhangZhiqin Chu
Jun 13, 2019·Accounts of Chemical Research·Alina SigaevaRomana Schirhagl
Aug 21, 2021·Nanotechnology·Masazumi Fujiwara, Yutaka Shikano

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