PMID: 6972531Jan 1, 1981Paper

Measurement of mammalian 25-hydroxyvitamin D3 24R-and 1 alpha-hydroxylase

Proceedings of the National Academy of Sciences of the United States of America
Y Tanaka, H F DeLuca

Abstract

An in vitro assay of mammalian 25-hydroxyvitamin D3 1 alpha- and 24R-hydroxylases in kidney has been developed. It had been suggested that 25-hydroxyvitamin D binding protein present in mammalian blood and tissues inhibits the enzyme activities in cell-free preparations by binding the substrate 25-hydroxyvitamin D3 more strongly than the hydroxylases bind it. This inhibitory effect is overcome by the addition of substantial amounts of unlabeled 25-hydroxyvitamin D3 to saturate the binding sites of this protein. The resulting metabolites produced in vitro by rat kidney homogenates were isolated and firmly identified by ultraviolet absorption spectrometry and mass spectrometry as 1,25-dihydroxyvitamin D3 and (24R)-24,25-dihydroxyvitamin D3. Maximal 1 alpha-hydroxylation of 25-hydroxyvitamin D3 could be demonstrated in kidney homogenates prepared from vitamin D-deficient rats. Thyroparathyroidectomy of these rats resulted in total suppression of the 1 alpha-hydroxylase. Homogenates of kidney from rats given vitamin D showed little or no 1 alpha-hydroxylase and substantial 24R-hydroxylase activity. Thyroparathyroidectomy of these rts markedly increased the 24R-hydroxylase activity.

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Citations

Jan 1, 1988·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·M IshidaY Seino
Jul 4, 2006·Journal of Bone and Mineral Metabolism·Tadatoshi SatoEiji Takeda
Jan 1, 1985·Comparative Biochemistry and Physiology. A, Comparative Physiology·H S Tenenhouse
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·N IkekawaH F DeLuca
Jan 10, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T TaniguchiT Setoguchi
Nov 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·C ZieroldH F DeLuca
Jul 28, 2015·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Perla C Reyes-Fernandez, James C Fleet
Mar 29, 2011·Clinics and Research in Hepatology and Gastroenterology·Silvia ZúñigaNicolas Chignard
Oct 1, 1990·Analytical Biochemistry·M Burgos-TrinidadH F DeLuca
Aug 1, 1981·Archives of Biochemistry and Biophysics·R T FranceschiH F DeLuca
Sep 28, 1984·Biochemical and Biophysical Research Communications·I Holmberg
Feb 1, 1982·Archives of Biochemistry and Biophysics·Y Tanaka, H F DeLuca
Jul 31, 2010·Human & Experimental Toxicology·Minfen YanShifang Weng
Jan 10, 2003·Journal of Cellular Biochemistry·Claudia ZieroldHector F DeLuca
Aug 24, 2018·Molecular Medicine·Mark Simon SteinLeonard Charles Harrison
Jan 11, 2013·Physiological Reviews·Sascha Kopic, John P Geibel
Jun 15, 2019·FEBS Letters·Ludmilla BärMichael Föller
Nov 12, 2019·Frontiers in Endocrinology·Rene F ChunJohn S Adams
Jun 13, 2019·Clinical Chemistry and Laboratory Medicine : CCLM·Elisa De PaolisEttore Capoluongo
May 1, 1990·The American Journal of Physiology·R ViethK H Norwich
Feb 1, 1984·The American Journal of Physiology·Y Tanaka, H F DeLuca
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Jun 11, 2002·Archives of Biochemistry and Biophysics·Matthew J Beckman, Hector F DeLuca
May 13, 2021·Current Developments in Nutrition·Matthew F Warren, Kimberly A Livingston
Mar 1, 1983·Analytical Biochemistry·B Lobaugh, M K Drezner
Nov 1, 1988·Kidney International·K SatomuraH F DeLuca
Jan 1, 1984·Kidney International·J CunninghamL V Avioli

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