PMID: 6173884Jan 1, 1982

Measurement of metabolites of dopamine and 5-hydroxytryptamine in cerebroventricular perfusates of unanesthetized, freely-moving rats: selective effects of drugs

Pharmacology, Biochemistry, and Behavior
J A Nielsen, K E Moore

Abstract

A method was developed for measuring endogenous acid metabolites of dopamine (dihydroxyphenylacetic acid, DOPAC; homovanillic acid, HVA) and of 5-hydroxytryptamine (5-hydroxyindoleacetic acid, 5HIAA) in serial samples of cerebroventricular perfusates from unanesthetized, freely-moving rats by the use of high performance liquid chromatography with electrochemical detection. Systemic administration of probenecid increased while pargyline reduced the efflux of all of the acid metabolites. Intraperitoneal injection of L-tryptophan increased the perfusate content of 5HIAA, decreased HVA and was without effect on DOPAC. Injections of haloperidol increased the efflux of DOPAC and HVA but did not alter the efflux of 5HIAA. Administration of apomorphine decreased the concentration of all three compounds. These drug-induced changes are generally consistent with those reported to occur in brain tissues. The results indicate that this method can be employed to monitor the activity of dopamine and 5-hydroxytryptamine neurons in unanesthetized, freely-moving rats.

References

Jun 23, 1978·Brain Research·M L Aizenstein, J Korf
Aug 12, 1977·Brain Research·R M WightmanR N Adams
Jun 1, 1980·Pharmacology, Biochemistry, and Behavior·C C LoullisM H Aprison
Apr 1, 1964·International Journal of Neuropharmacology·B E ROOSB WERDINIUS

Citations

Jan 1, 1983·Journal of Neural Transmission·J L ElghoziK H Le Quan-Bui
Dec 1, 1989·Pharmacology, Biochemistry, and Behavior·K IwasakiS Ueki
Jun 1, 1983·Brain Research Bulletin·C D Blaha, R F Lane
Jan 1, 1986·Annals of the New York Academy of Sciences·P H HutsonG Curzon
Jun 29, 2017·Journal of Neural Transmission·Erik PålssonMikael Landén

Related Concepts

Metazoa
Blood - Brain Barrier Anatomy
Brain
Intropin
Homovanillic Acid
Hydroxyindoleacetic Acid
Pargyline Hydrochloride
Perfusion
Pro-Cid
August Rats

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