PMID: 15387408Sep 25, 2004Paper

Measurement of NF-kappaB in normal and reconstructed human skin in vitro

Journal of Materials Science. Materials in Medicine
T SunSheila MacNeil

Abstract

The survival of grafted donor skin for the treatment of burn injuries depends on several factors including wound bed vascularisation and the intensity of acute inflammation shortly after injury. However, acceptance rates approximate 50% at best and therefore a clinical need exists for improvement. The aim of the study was to develop a method for assessing the inflammatory response of cells in skin tissue based on activation of the NF-kappa B (NF-kappaB) transcription factor complex, thereby providing a basis for analysing the inflammatory component and anti-inflammatory strategies for tissue-engineered treatments. We have extended a standard method of measuring NF-kappaB in monolayer cultures that relies on determining translocation of the p65 subunit from the cytoplasm to the nucleus. Normal human skin and tissue engineered skin was analysed using an immunofluorescence microscopy technique, that revealed base line NF-kappaB activation in the epidermis and dermis were different. It was possible to determine the activation of NF-kappaB in skin tissue, enabling correlation that NF-kappaB measurement is a sensitive indicator of cellular responses in 3-D tissue. The approach will provide a basis for early responses of skin cells in...Continue Reading

Citations

Mar 23, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Sarah D Lamore, Georg T Wondrak
Dec 16, 2011·Journal of Tissue Engineering and Regenerative Medicine·T SunM O Riehle
Sep 5, 2009·Biotechnology and Bioengineering·Irene CantónSheila MacNeil
Feb 4, 2011·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·H L MorrisG C Reilly

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