Measurement of NO and NO synthase

Current Protocols in Neuroscience
B A Weissman, S S Gross

Abstract

Nitric oxide (NO) is a key biosignaling molecule produced in both peripheral tissues and the central nervous system by a family of enzymes known as nitric oxide synthases (NOSs). NOSs convert L-arginine to stoichiometric quantities of NO and L-citrulline using molecular oxygen and NADPH as cofactors. Techniques for measurement of NO and NOS activity are essential to demonstrate the role of NO and NO-derived species in biological systems. This unit describes two methods for detection of NO: a direct method employing chemiluminescent detection and one based on quantification of the stable oxidation products with detection using the Griess reagent. Additionally, NOS activity can be quantified by measuring the conversion of radiolabeled L-arginine to radiolabeled L-citrulline.

References

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Citations

Nov 19, 2013·PloS One·Jérôme RobertSimon P Hoerstrup
Jul 25, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ji-Yeon YuSeikwan Oh
Jan 11, 2017·Antioxidants & Redox Signaling·Gopi K KolluruChristopher G Kevil
May 21, 2019·Journal of Cardiovascular Pharmacology and Therapeutics·Nour A Al-SawalhaOmar F Khabour
Jan 15, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Mark J CrabtreeSteven S Gross
May 15, 2013·Acta Diabetologica·F RaffaelliL Nanetti

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