Measurement of nucleic acid concentrations using the DyNA Quant and the GeneQuant

BioTechniques
J M TeareC Grabau

Abstract

Molecular biology is now a routine tool in almost all biological research fields. With the exponential growth in the number of molecular biological techniques, there is a recognizable need for sensitive, accurate and precise quantitation of nucleic acids. We present here two complementary instruments designed for the quantitation of nucleic acids, the GeneQuant II and the DyNA Quant 200 Fluorometer. The GeneQuant II can rapidly determine the UV absorbance of a solution and display the calculated DNA, RNA or protein concentration. In addition, the GeneQuant can display calculated melting temperatures for a given DNA oligonucleotide base sequence, a useful feature for primer design. The DyNA Quant 200 quantitates DNA on the basis of the fluorescent Hoechst 33258 dye/double-stranded (ds)DNA assay. Upon binding to dsDNA, the spectral properties of the dye change such that it becomes highly fluorescent at 460 nm when excited at 365 nm. The assay has proven to be a specific and sensitive alternative method for DNA quantitation, particularly for unpurified DNA samples. Together, the GeneQuant II and the DyNA Quant 200 are a cost-effective and convenient solution to the routine protein and nucleic acid quantification needs of the molec...Continue Reading

Citations

May 11, 2007·Expert Opinion on Drug Delivery·Mayank D Bhavsar, Mansoor M Amiji
Oct 17, 2013·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·J PremanandhM Maruthamuthu
Mar 20, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·Maria Paula Cabral CampelloFernanda Marques
Mar 18, 2009·Biochemical Society Transactions·Rupak DoshiNicola Tirelli
Aug 29, 2006·Biotechnology Letters·Johanna LeggateBurton W Blais
Dec 19, 2009·Expert Opinion on Drug Delivery·Maria Luisa Bondì, Emanuela Fabiola Craparo
Jan 18, 2017·Scientific Reports·Lanhui LiLingling Shui
Aug 20, 2019·Life Sciences in Space Research·Kianoosh PeyvanAndrew Pohorille
Feb 16, 2010·Chemical Reviews·Jean-Claude G Bünzli
Feb 1, 2000·Biochemical and Biophysical Research Communications·L H HoP D Zalewski
Jun 18, 2017·Journal of Inorganic Biochemistry·Nádia RibeiroIsabel Correia
Feb 7, 2008·Archives of Pathology & Laboratory Medicine·Jennifer L Hunt

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