PMID: 7336524Jan 1, 1981Paper

Measurement of partial specific thickness (net thickness) of critical-point-dried cultured fibroblast by energy analysis

Ultramicroscopy
J HosoiK Hama

Abstract

The relative specimen thickness obtained with an energy analyser is one of the most important parameters in electron microscopy. It has been made clear that this parameter can be applied not only to inorganic specimens, but also to organic and biological specimens. Moreover, the partial specific thickness of a critical-point-dried cultured fibroblast, that is, a net thickness converted in terms of Epon, was calculated from the relative specimen thickness. The partial specific thickness of each domain of the critical-point-dried cultured fibroblast is: CC 0.05 microns, PE 0.14 microns, ED 0.32 microns and PN 0.54 microns. As a result, it is suggested that 27% of the volume of this specimen consists of biological materials.

Citations

Dec 12, 2002·Micron : the International Research and Review Journal for Microscopy·Yolande BertaZhong L Wang
Aug 1, 1983·Quarterly Reviews of Biophysics·T A Hall, B L Gupta
Mar 13, 2014·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Aycan YurtseverDavid A Muller
Dec 28, 2010·Ultramicroscopy·Na NiChris Grovenor
May 1, 1988·Journal of Electron Microscopy Technique·K Zierold
Nov 1, 1984·Journal of Microscopy·T A Hall, B L Gupta

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