PMID: 9540797Apr 16, 1998Paper

Measurement of peptide aggregation with pulsed-field gradient nuclear magnetic resonance spectroscopy

Biochimica Et Biophysica Acta
S L MansfieldC K Larive

Abstract

Interactions between hydrophobic patches in proteins are often a driving force for denaturation and aggregation. The aggregation of the beta-amyloid peptide fragment, VHHQKLVFFAEDVGSNK (beta(12-28)), has been investigated in aqueous solution at low pH. This peptide contains a central hydrophobic patch spanning residues 17-21. Diffusion coefficients measured with pulsed-field gradient NMR as a function of peptide solution concentration were used to assess the extent of aggregation. Following the hypothesis that hydrophobic interactions are an important driving force in the aggregation of this peptide at low pH, a non-aggregating analog of the beta(12-28) peptide, [Gly19,20]beta(12-28) was synthesized. In the [Gly19,20]beta(12-28) peptide, the replacement of the two phenylalanine residues disrupts the hydrophobic interactions which drive the aggregation of beta(12-28). The diffusion coefficient of the [Gly19,20]beta(12-28) peptide is invariant over the concentration range studied and provides a good estimate of the monomeric diffusion coefficient of beta(12-28). A second peptide analog was synthesized in which the phenylalanine at position 20 was replaced with a cysteine residue. The disulfide-linked dimer, ([Cys20]beta(12-28))2,...Continue Reading

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Citations

Jun 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Tobin J Dickerson, Kim D Janda
Jul 23, 2013·Biophysical Journal·Annie V RaditsisGiuseppe Melacini
Apr 29, 2009·Journal of Peptide Science : an Official Publication of the European Peptide Society·Dimitra BenakiEmmanuel Mikros
Dec 27, 2013·Biochimica Et Biophysica Acta·Mario ScrimaAnna Maria D'Ursi
Aug 15, 2000·Journal of Structural Biology·R M Murphy, M M Pallitto
Dec 31, 2010·Biophysical Journal·Julijana Milojevic, Giuseppe Melacini
Mar 17, 2015·The Journal of Chemical Physics·Adrian A Hernandez SantiagoMaxim P Evstigneev
Jul 13, 2016·The Journal of Physical Chemistry. B·Debasis BanikNilmoni Sarkar
Nov 24, 2007·Biotechnology Journal·Steven S-S WangHwai-Shen Liu
Jul 11, 2006·Magnetic Resonance in Chemistry : MRC·Jens DanielssonAstrid Gräslund
Nov 27, 2002·Journal of Peptide Science : an Official Publication of the European Peptide Society·Francesc RabanalErnest Giralt
Dec 25, 2003·Journal of Biomolecular Structure & Dynamics·Tracy L WhiteheadRickey P Hicks
Sep 20, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Y YuQ-S Zheng
Nov 3, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·M Aoudia, M A J Rodgers
Jul 17, 1999·Analytical Chemistry·C K LariveS Bogdanowich-Knipp
Jul 28, 2005·Journal of the American Chemical Society·Yingjie LiJean Baum
Aug 6, 2003·Biochemistry·Steven S-S WangErik J Fernandez
Jun 30, 2005·Journal of the American Chemical Society·Veronica EspositoGiuseppe Melacini

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