PMID: 3214675Sep 1, 1988Paper

Measurement of phenylacetic acid in cerebrospinal fluid and plasma using combined gas chromatography/electron capture chemical ionization mass spectrometry

Biomedical & Environmental Mass Spectrometry
K F FaullR J King

Abstract

Details are presented of an ultra-sensitive gas chromatographic/mass spectrometric assay for phenylacetic acid in plasma and cerebrospinal fluid based on measurements of the relative intensities of the carboxylate anions, derived from the penta- and tetrafluorobenzyl esters under electron capture chemical ionization conditions, of unlabeled and a (13C2)-labeled internal standard. The limits of detection for the penta- and tetrafluorobenzyl esters are 0.85 and 4.0 pg respectively, and the assay is capable of measuring phenylacetic acid concentrations in samples as small as 20 microliter of CSF and plasma. The penta- and tetrafluorobenzyl esters are chromatographically separated on the gas chromatograph column, which allows for their co-injection and independent measurement from the same chromatogram.

References

Dec 16, 1978·Lancet·M SandlerR Matthews
Aug 1, 1978·Biomedical Mass Spectrometry·L E FellowsM Sandler
Jan 1, 1975·Canadian Journal of Biochemistry·P H Wu, A A Boulton
Aug 4, 1975·Clinica Chimica Acta; International Journal of Clinical Chemistry·B L GoodwinM Sandler
May 31, 1985·Journal of Chromatography·E J PenningsG M van Kempen
Nov 4, 1985·Life Sciences·J D ElsworthM Sandler
Jul 25, 1972·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·M O JamesM Reidenberg
Mar 1, 1972·Clinica Chimica Acta; International Journal of Clinical Chemistry·H C CurtiusK Baerlocher
Dec 1, 1983·Journal of Chromatographic Science·F KaroumR J Wyatt
Jan 1, 1984·Analytical Biochemistry·F GusovskyJ I Javaid
Jun 15, 1984·Biochemical Pharmacology·A D MosnaimU P Madubuike
Aug 1, 1983·Journal of Clinical Psychopharmacology·H SabelliJ Javaid
Jun 1, 1982·Journal of Neurochemistry·D A Durden, A A Boulton

❮ Previous
Next ❯

Citations

Nov 1, 1989·Psychiatry Research·K F FaullJ G Csernansky
Jan 7, 1998·Journal of Neurology, Neurosurgery, and Psychiatry·G ZhouT Matsuishi
Dec 1, 1990·Journal of Neuroscience Research·K F FaullS G Wiener
Dec 1, 1991·Biochemical Medicine and Metabolic Biology·F A Hommes

❮ Previous
Next ❯

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.