Measurement of plasma gamma-glutamyltransferase in clinical chemistry: kinetic basis and standardisation propositions

Clinica Chimica Acta; International Journal of Clinical Chemistry
F SchieleG Siest

Abstract

The conditions for the measurement of gamma-glutamyltransferase (EC 2.3.2.2) activity of human plasma were studied at 30 degrees C using the kinetic technique of Szasz [3]. The optimum pH in Tris (hydroxymethylaminomethane) buffer and 2-amino-2-methyl-1.3-propanediol at a concentration of 100 mmol/1 are 8.0 and 8.1. The kinetic characteristics of human plasma gamma-glutamyltransferase were studied using gamma-L-glutamyl p-nitroanilide and its carboxyl derivative as donor substrates. Glycylglycine was chosen as the best acceptor of the gamma-glutamyl radical. Under these conditions, we have shown that the inhibition by the donor substrate was more important at acidic pH and vanished at alkaline pH. This inhibition was obviously related to the presence of the acceptor, but did not vary with glycylglycine concentration. At pH 8.0, by increasing the acceptor concentration some competition occurs at the donor binding site, as reported by other authors in relation to the known ping-pong bi-bi enzyme mechanism for the gamma-glutamyltransferase. Some displacement of donor substrate by increasing amounts of acceptor substrate could be observed at all pH values we studied. However, the influence of glycylglycine on the enzyme's maximum v...Continue Reading

References

Sep 20, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·M CucuianuA Opincaru
Oct 15, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·L Theodorsen, J H Stromme
Dec 1, 1974·Scandinavian Journal of Clinical and Laboratory Investigation·N E Huseby, J H Strömme
Apr 14, 1981·Biochimica Et Biophysica Acta·D BagrelG Siest

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Citations

Jul 15, 1988·Clinica Chimica Acta; International Journal of Clinical Chemistry·P M VerhoeffJ C Hafkenscheid
Mar 31, 1988·Clinica Chimica Acta; International Journal of Clinical Chemistry·G SiestM Wellman
Aug 9, 2003·Biochemical Pharmacology·Sandrine DaubeufAthanase Visvikis
Nov 26, 2011·Science Translational Medicine·Yasuteru UranoHisataka Kobayashi
Nov 13, 2008·Archives of Biochemistry and Biophysics·Valeria AngeliEmilia Bramanti
Feb 20, 2007·Biochemical and Biophysical Research Communications·Kyung-Mi KimSeung-Chul Baik
Oct 29, 1982·Biochemical and Biophysical Research Communications·M LetellierC PetitClerc
Jul 19, 2002·Biochemical Pharmacology·Sandrine DaubeufAthanase Visvikis
Dec 29, 2004·Free Radical Research·Antoni SuredaAntoni Pons
Dec 8, 2016·Breastfeeding Medicine : the Official Journal of the Academy of Breastfeeding Medicine·Mariela Valentina Cortez, Elio Andrés Soria
Dec 20, 2003·Biochemical and Biophysical Research Communications·Sandrine DaubeufAthanase Visvikis

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