Abstract
Work to date has identified prostate-specific membrane antigen (PSMA) as a membrane-bound glycoprotein with high specificity for prostatic epithelial cells. PSMA reacts with the monoclonal antibody 7E11.C5, which is present in serum, seminal fluid, and prostatic epithelial cells, and is increased in its expression in the presence of a hormone refractory state associated with prostatic cancer. This report confirms these results and further documents the presence of the monoclonal antibody 3F5.4G6, which reacts with the extracellular domain of PSMA. This region of PSMA is also an element present in a truncated version of the protein, so-called PSM'. Immune precipitation with either 7E11.C5 or 3F5.4G6 yields an isolated protein species that are reactive with the reciprocal antibody in Western blot analysis. Thus, 3F5.4G6 recognizes the same PSMA protein as does 7E11.C5, but at different epitopes on essentially opposite ends of the molecule. These two antibodies are well suited for use in a sandwich immunoassay, either one as a capture or detection antibody. Current work on this is underway. This report also confirms that 7E11.C5 Western blots for PSMA are negative with normal human brain tissue. The monoclonal antibody 9H10 does n...Continue Reading
References
Apr 1, 1983·Molecular Immunology·T P Hopp, K R Woods
Jan 1, 1981·Methods in Enzymology·G Galfrè, C Milstein
Mar 1, 1995·The Prostate·G P MurphyR J Barren
Sep 4, 1995·International Journal of Cancer. Journal International Du Cancer·J K TroyerG L Wright
Oct 1, 1994·The Prostate·Y P RochonG P Murphy
Mar 1, 1994·Journal of Cellular Physiology·D B Pelletier, A L Boynton
Jan 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·R E CarterJ T Coyle
Jan 1, 1995·Urologic Oncology·J K TroyerG L Wright
Citations
Aug 22, 2000·Hybridoma·W T TinoE H Holmes
Feb 28, 2001·Expert Opinion on Investigational Drugs·E H Holmes
Nov 17, 1998·The Journal of Urology·G P MurphyE H Holmes
May 15, 2001·Genomics·F WattP L Molloy
Jun 2, 2000·Protein Expression and Purification·Z XiaoG L Wright
Jan 1, 1997·Urological Research·N S VerkaikJ C Romijn
Jun 1, 1997·The Prostate·X GaoK V Honn
Jun 4, 1998·Cancer·D G BostwickG P Murphy
Aug 3, 2004·The Prostate·Qiangzhong MaRichard P Junghans
Aug 15, 1996·Cancer·G P MurphyE H Holmes
Feb 15, 2001·The Prostate·M AnidjarO Cussenot
Mar 8, 2008·Leukemia·I HusJ Roliński
Jun 12, 1998·Journal of Surgical Oncology·M L Salgaller, P A Lodge
Apr 12, 2012·Macromolecular Bioscience·Jihua LiuJindřich Kopeček
May 6, 1999·European Journal of Immunology·L GranzieroA Vitiello
Jun 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·James V TricoliBarbara A Conley
Mar 1, 1997·Urology·W D Heston
Sep 10, 2018·Biosensors & Bioelectronics·Bo WeiZhugen Yang