Measurement of protein digestibility in humans by a dual-tracer method.

The American Journal of Clinical Nutrition
Sarita DeviAnura V Kurpad

Abstract

Recent evaluations of the risk of dietary protein deficiency have indicated that protein digestibility may be a key limiting factor in the provision of indispensable amino acids (IAAs), particularly for vulnerable populations living in challenging environments where intestinal dysfunction may exist. Since the digestion of protein occurs only in the small intestine, and the metabolic activity of colonic bacteria confounds measurements at the fecal level, there is a need to develop noninvasive protein digestibility measurements at the ileal level. We used a dual-tracer method with stable isotopes to characterize the digestibility of uniformly labeled [13C]-spirulina protein as a standard protein, in comparison to a mixture of 2H-labeled crystalline amino acids, and then demonstrated the use of this standard protein to measure the digestibility of selected legumes (chick pea and mung bean) through the use of proteins that were intrinsically labeled with 2H. The digestibility of uniformly labeled [13C]-spirulina was first measured in 6 healthy volunteers (3 males and 3 females) by feeding it along with a standard mixture of 2H-labeled amino acids, in a dual-tracer, plateau-fed test meal approach. Next, intrinsically labeled legume ...Continue Reading

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Citations

Mar 29, 2019·The American Journal of Clinical Nutrition·Nirupama ShivakumarAnura V Kurpad
Sep 19, 2019·The Journal of Nutrition·Paul J Moughan, Robert R Wolfe
Jun 5, 2020·The Proceedings of the Nutrition Society·Barbara A FieldingKatherine Pinnick
Sep 2, 2020·European Journal of Nutrition·Romain TessierClaire Gaudichon
Feb 1, 2020·Animal Frontiers : the Review Magazine of Animal Agriculture·Teena DasiBharati Kulkarni
Jun 14, 2019·Current Opinion in Clinical Nutrition and Metabolic Care·Mariëlle P K J EngelenNicolaas E P Deutz
Aug 3, 2019·The American Journal of Clinical Nutrition·Sindhu KashyapAnura V Kurpad
Jul 25, 2020·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Wilfredo D MansillaAnna K Shoveller
Apr 16, 2019·The American Journal of Clinical Nutrition·Rebecca KuriyanAnura V Kurpad
Oct 8, 2020·The Journal of Nutrition·Katia CaballeroRajavel Elango
Oct 24, 2020·Current Opinion in Clinical Nutrition and Metabolic Care·Claire Gaudichon, Juliane Calvez
Jan 26, 2021·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·M S BrookP J Atherton
Mar 8, 2021·The American Journal of Clinical Nutrition·Sindhu KashyapAnura V Kurpad
Apr 21, 2021·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Sarah K KirschnerMariëlle P K J Engelen
May 4, 2021·Critical Reviews in Food Science and Nutrition·Zuhaib F BhatHina F Bhat
May 23, 2021·JPEN. Journal of Parenteral and Enteral Nutrition·Sarah K KirschnerMariëlle P K J Engelen
Sep 14, 2021·Sports Medicine·Philippe J M PinckaersLuc J C van Loon
Dec 6, 2021·The Journal of Nutrition·Daniel J HoffmanTeresa A Davis

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