PMID: 4881437Jan 1, 1969Paper

Measurement of rates of phagocytosis: the use of cellular monolayers

The Journal of Cell Biology
R H MichellM L Karnovsky

Abstract

A method has been developed for measuring the rate of phagocytosis rather than the quantity of particles ingested per cell when the process is virtually complete. The method, which is simpler and more rapid than those described previously, utilizes cellular monolayers, radioactive particles, and short incubation times. Under the conditions described, the rate of uptake of particles by either guinea-pig peritoneal or human blood leukocytes was proportional to both cell concentration and the time of incubation, and was independent of changes in the concentration of particles during the measurement. The particles were retained by the cells for at least 90 min. The most suitable particles so far used have been (32)P-labeled Salmonella typhimurium, and acetyl-(14)C- or methyl-(14)C-labeled starch particles. The oxidation of (14)C-labeled glucose has been studied under the same conditions that were used for the assays of phagocytosis: the greatest increase in formation of (14)CO(2) from glucose-1-(14)C occurred a few minutes after the most rapid period of phagocytosis.

References

Feb 1, 1967·Biochemistry·R A Weisman, E D Korn
May 1, 1967·The Journal of Clinical Investigation·S E Malawista, P T Bodel
Sep 1, 1959·The Journal of Experimental Medicine·Z A COHN, S I MORSE
Jun 1, 1963·The Journal of Cell Biology·R ORENM L KARNOVSKY

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Citations

Jan 1, 1986·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·I GriersonA Ahmed
Apr 17, 1986·Journal of Immunological Methods·T Oda, H Maeda
Jan 1, 1990·International Journal of Immunopharmacology·R R IyerD N Rao
Mar 1, 1995·International Journal of Immunopharmacology·M PolanskiM L Karnovsky
Apr 11, 1974·The New England Journal of Medicine·T P Stossel
May 2, 1974·The New England Journal of Medicine·R I Handin, T P Stossel
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J D LoikeJ M Sturtevant
May 1, 1973·Archives of Environmental Health·C L VassalloJ B Gee
Sep 1, 1973·The Journal of Cell Biology·S Githens, M L Karnovsky
Nov 1, 1973·The Journal of Cell Biology·J P Cox, M L Karnovsky
Oct 1, 1977·The Journal of Cell Biology·M T Mazur, J R Williamson
Jun 1, 1969·The Journal of Experimental Medicine·R B JohnstonF S Rosen
Jun 1, 1971·The Journal of Experimental Medicine·C F NathanJ R David
Feb 1, 1973·The Journal of Experimental Medicine·M StubbsM L Karnovsky
Jul 1, 1973·The Journal of Experimental Medicine·S R Simmons, M L Karnovsky
Jan 1, 1975·The Journal of Experimental Medicine·D B Drath, M L Karnovsky
Nov 1, 1987·Annals of Surgery·P T RoughneenB J Rowlands
Jan 1, 1992·Microbiology and Immunology·S NagaoY Tanigawa
Jun 1, 1979·European Journal of Clinical Investigation·M TorresM A Gougerot
Sep 1, 1990·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·R J Foerster, G Wolf
Jun 13, 1975·Annals of the New York Academy of Sciences·M L KarnovskyA Harper
Jun 1, 1974·The Journal of Clinical Investigation·L A Boxer, T P Stossel
Jun 1, 1974·The Journal of Clinical Investigation·J T Curnutte, B M Babior
Aug 1, 1981·The Journal of Clinical Investigation·S K Adeniyi-Jones, M L Karnovsky
Apr 1, 1978·Journal of Cellular Physiology·D B DrathG L Huber
Feb 1, 1972·Journal of Cellular Physiology·J NoseworthyM L Karnovsky
Nov 1, 1978·Kidney International·Z SharonE J Lewis
Dec 8, 2015·Nano Today·Heather Herd GustafsonHamidreza Ghandehari
Jan 1, 1980·British Journal of Diseases of the Chest·H E LarsonD A Tyrrell
Dec 1, 1982·Experimental Cell Research·W NoskeK Keller

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